Protein Info for BPHYT_RS14360 in Burkholderia phytofirmans PsJN

Annotation: DNA repair protein RecO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 309 PF11967: RecO_N" amino acids 78 to 150 (73 residues), 75.2 bits, see alignment E=3.4e-25 TIGR00613: DNA repair protein RecO" amino acids 80 to 223 (144 residues), 86.4 bits, see alignment E=9.9e-29 PF02565: RecO_C" amino acids 157 to 294 (138 residues), 52.2 bits, see alignment E=6.1e-18

Best Hits

Swiss-Prot: 96% identical to RECO_PARXL: DNA repair protein RecO (recO) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K03584, DNA repair protein RecO (recombination protein O) (inferred from 100% identity to bpy:Bphyt_2897)

Predicted SEED Role

"DNA recombination and repair protein RecO" in subsystem DNA repair, bacterial RecFOR pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SZV3 at UniProt or InterPro

Protein Sequence (309 amino acids)

>BPHYT_RS14360 DNA repair protein RecO (Burkholderia phytofirmans PsJN)
MGTNDAWMTLNSDTDPDDSEPAAPARDLSKPASKPARSTRKSSSAKSAQGAEGAEGEPRK
TPTRRAPRISASDYRIAEQPAFVLHSYPYRETSLIIDVLSRDHGRLALVAKGAKRPHSAL
RGVLQTFQPLALSWSGKSEVRTLTGAEWVGGMLPLAGDALLCGFYVNELLVKFCAREDPH
PQLFHHYVVTMTRLAHDEPPVQVLRSFERVLLRETGYAMALDRTVARKAVLADGRYVFDP
ERGVREASDDLPALWPVIAGQTLLDMEQDDYHRAQTVAQSKTLMRFLLNTYLGGTPLATR
QILIDLQNL