Protein Info for BPHYT_RS14110 in Burkholderia phytofirmans PsJN

Annotation: polyphosphate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 687 TIGR03705: polyphosphate kinase 1" amino acids 7 to 682 (676 residues), 954.9 bits, see alignment E=1.3e-291 PF13089: PP_kinase_N" amino acids 8 to 112 (105 residues), 114.7 bits, see alignment E=6.3e-37 PF02503: PP_kinase" amino acids 122 to 304 (183 residues), 188.6 bits, see alignment E=2.3e-59 PF17941: PP_kinase_C_1" amino acids 331 to 497 (167 residues), 240.3 bits, see alignment E=2.1e-75 PF13091: PLDc_2" amino acids 374 to 495 (122 residues), 32.9 bits, see alignment E=1.3e-11 PF13090: PP_kinase_C" amino acids 509 to 678 (170 residues), 209.9 bits, see alignment E=4.8e-66

Best Hits

Swiss-Prot: 100% identical to PPK1_PARPJ: Polyphosphate kinase (ppk) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 100% identity to bpy:Bphyt_2851)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.1

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SZQ7 at UniProt or InterPro

Protein Sequence (687 amino acids)

>BPHYT_RS14110 polyphosphate kinase (Burkholderia phytofirmans PsJN)
MSIRYPLLNRELGILGFNERVLAQAADPAVPLLERLRFICITSSNLDEFFEVRMAGLQEQ
MRDNPGALSPDGMSLQHVYDLVVERAQKLVHRQYTMLHDTVLTALEAEGIYFHGTEAWNE
AQTEWARNYFFDELLPVLTPIGLDPAHPFPRVLNKSLNFVVELEGKDAFGRQAMMGIVQA
PRALPRLVRMPQELSGYPHGFVLLSSLLQRFVGELFPNLVVRSCNQFRITRNSELFVDED
EITNLRVALQGELPARHLGNAVRLEVSAETPTHVVRRLLDESGLSDKDCYYADGPVNLVR
LMQLPEMVDRPDLKFVPHIPAIPAQVANSVSMFDVIDQGDVLLHHPYESFQPVLELLLQA
AKDPNVVAIKQTIYRTGTDSPLMDALMQAARNGKEVTVVVELLARFDEETNINWASQLEA
VGAHVVYGVVGHKCHAKMMLIVRRVSVGGKTTLKRYVHLGTGNYHPRTARLYTDFGLMTA
DQKICEDVHHVFQQLTGIGGELKLHELWQSPFTLHPKLVEAIRAEAEHARAGKKARIVAK
MNALLEPTVIAELYEAAQAGVKIDLIVRGVCSLQPGVAGLSENITVRSIVGRFLEHHRIF
YFYDGGKEQVYLSSADWMDRNFFRRVEVAFPINNRRLKRRVIAEGLSAFLGDNQSAWLMQ
SDGHYRRRRPGKSSRNAQMSLLGKFCS