Protein Info for BPHYT_RS13825 in Burkholderia phytofirmans PsJN

Annotation: Fosmidomycin resistance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 419 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 34 to 59 (26 residues), see Phobius details amino acids 69 to 90 (22 residues), see Phobius details amino acids 111 to 141 (31 residues), see Phobius details amino acids 162 to 184 (23 residues), see Phobius details amino acids 191 to 212 (22 residues), see Phobius details amino acids 235 to 254 (20 residues), see Phobius details amino acids 274 to 294 (21 residues), see Phobius details amino acids 304 to 323 (20 residues), see Phobius details amino acids 329 to 349 (21 residues), see Phobius details amino acids 361 to 383 (23 residues), see Phobius details amino acids 390 to 409 (20 residues), see Phobius details PF07690: MFS_1" amino acids 42 to 275 (234 residues), 105.4 bits, see alignment E=1.6e-34 amino acids 251 to 415 (165 residues), 51.5 bits, see alignment E=3.8e-18

Best Hits

Swiss-Prot: 70% identical to FSR_ECOLI: Fosmidomycin resistance protein (fsr) from Escherichia coli (strain K12)

KEGG orthology group: K08223, MFS transporter, FSR family, fosmidomycin resistance protein (inferred from 100% identity to bpy:Bphyt_2794)

MetaCyc: 70% identical to fosmidomycin efflux pump (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-41

Predicted SEED Role

"Fosmidomycin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SZK0 at UniProt or InterPro

Protein Sequence (419 amino acids)

>BPHYT_RS13825 Fosmidomycin resistance protein (Burkholderia phytofirmans PsJN)
METSLDKSALAGGAGTGTGSAAAAQPAAKAQRTVYSVLGAISFSHLLNDMIQSLILAIYP
MLKDNFSLSFGQIGLITLTYQITASLLQPLVGSYTDKHPKPYSLPVGMGFTLAGLLLMSV
APSFGVLLVAAALVGCGSSVFHPESSRVARMASGGRHGLAQSLFQVGGNAGSSLGPLLAA
LIVIPHGQRSIAWFSVAALVAIVVLTQIGRWYKAHPSVKKARSVAGHATLSRNKVMFAMG
VLILLVFSKYFYLASINSYFTFYLIDKFHLPVQAAQVHLFVFLAAVAAGTVIGGPIGDRI
GRKYVIWVSILGVAPFTLLLPYANLFWTGVLTVIIGVVLASAFSAILVYAQELIPGKVGM
VAGLFFGFAFGMGGIGAAVLGQLADATSITYVYKVCSFLPLIGVLTVFLPDVEGKRAKA