Protein Info for BPHYT_RS12205 in Burkholderia phytofirmans PsJN

Annotation: N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 294 TIGR03533: protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific" amino acids 6 to 288 (283 residues), 412.8 bits, see alignment E=7.4e-128 TIGR00536: methyltransferase, HemK family" amino acids 7 to 263 (257 residues), 224.8 bits, see alignment E=1.2e-70 PF06325: PrmA" amino acids 127 to 201 (75 residues), 31 bits, see alignment E=6.4e-11 PF05175: MTS" amino acids 128 to 209 (82 residues), 57 bits, see alignment E=7.1e-19 PF13847: Methyltransf_31" amino acids 129 to 212 (84 residues), 29 bits, see alignment E=2.9e-10 PF13649: Methyltransf_25" amino acids 130 to 201 (72 residues), 37.3 bits, see alignment E=1.3e-12 PF01170: UPF0020" amino acids 149 to 207 (59 residues), 22.3 bits, see alignment E=3.3e-08

Best Hits

Swiss-Prot: 77% identical to PRMB_BURPS: 50S ribosomal protein L3 glutamine methyltransferase (prmB) from Burkholderia pseudomallei (strain K96243)

KEGG orthology group: K07320, putative adenine-specific DNA-methyltransferase [EC: 2.1.1.72] (inferred from 100% identity to bpy:Bphyt_2470)

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p"

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T5K9 at UniProt or InterPro

Protein Sequence (294 amino acids)

>BPHYT_RS12205 N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase (Burkholderia phytofirmans PsJN)
MTLPFSTVRDLLRFAVSRFNQAELSFGHGSANAYDEAVYLVLHTLHLPLDLLDPFLDARL
TAAEIDAVLNVIERRATERVPAAYITQEAWMHGFRFHVDERVIVPRSFIGELLQDGLQPY
VEDPEQVSAVLELCTGSGCLAILAAHAFPNADIDAVDLSAPALEVAARNVADYKLDDRIA
LFEGDLYAPLAERRYDVIISNPPYVNAASMQDLPAEYKHEPNMALAGGVDGMDIVRRIIA
DARNWLTDEGVLVVEIGNERQHVEAAFGGLDLVWLSTSAGDDNVFLIQAADLPV