Protein Info for BPHYT_RS10585 in Burkholderia phytofirmans PsJN

Annotation: molecular chaperone DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 958 PF00012: HSP70" amino acids 141 to 357 (217 residues), 55.5 bits, see alignment E=3.1e-19 PF12531: DUF3731" amino acids 709 to 955 (247 residues), 361.3 bits, see alignment E=3.5e-112

Best Hits

Predicted SEED Role

"DnaK-related protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T4M5 at UniProt or InterPro

Protein Sequence (958 amino acids)

>BPHYT_RS10585 molecular chaperone DnaK (Burkholderia phytofirmans PsJN)
MKRYSVGIDLGTSNTVLAYAEAGSPGGSPLIHVFEIEQLVSPGEVAARPLLPSVRYQAAQ
GELNPGDLQLPWSGVAQGGARKDAQQDAQQDTPPVVIGRLARVLGAQVPGRLVSSAKSWL
SHASVDRTAPILPWGAADDVRKVSPVEASASYLAHVRAAWNQRFPDAPLERQDVVLTVPA
SFDEGARALTVEAARMAGLPTLKLLEEPQAAFYDWLFHHRERLASELADTRLVLICDVGG
GTTDLTLIEVGLRDGEPQLTRIGVGNHLMLGGDNMDLALAHLVEARLPGGSNQPGERARL
SAASLSQLVERCRNAKEQLLGAQAPDSTSITLLGAGSKLIGGARTAQVTRDEVERIIIDG
FFPAVASHERPGRPRGAIVEFGLPYATDAAVTRHIAAFLNRFAAQSRKALGVVEPSGSSD
EATLPVPDTLLLNGGVFRAEALTQRLASTLGTWRGEALNVLHNDNPDVAVARGAVAYSLA
RAGNAPKIGGGSPRSYFLVLDETGDGKPDAEPVQRGICLLPRGTEEGHEILIADRTFALR
LGHPVRFHLVSSSADTVYRPGELIDLAGGDFVRLPPIATIVQPRGAKSARETAVQIATSL
TEVGTLEVHCIELDDPTQRWLLEFQLRREHAQVNLTDDAAQSRNPALDQAIDHIDRCFGS
RAQKVDPKEVKRLRSQLEQLLGPRESWNSALLRELFGALWERARRRRRSADHERLWLNLA
GYCVRPGFGYPLDEWRVEQLWSLFDDGIQYVNESQVWSEWWTLWRRAAGGLDESAQLRVL
DAMTYLQGAALARHKLPFDVAKTGFTDMVRLSASLERIPVERKIELGESVLTRLKKPAEN
HQSWWAVGRIGARRPFYGSAHSVVPPEIAGAWLNAILALDWKKVDPAAFAAVQIARMTGD
RSRDLPEDLRLAVVRRLEAANAPRAWITMVSESVELDNADEGRVFGESLPAGLKLIAS