Protein Info for BPHYT_RS09840 in Burkholderia phytofirmans PsJN

Annotation: nitrite reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 846 PF07992: Pyr_redox_2" amino acids 1 to 282 (282 residues), 185.6 bits, see alignment E=4.2e-58 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 3 to 795 (793 residues), 1141.6 bits, see alignment E=0 PF00070: Pyr_redox" amino acids 145 to 222 (78 residues), 54.9 bits, see alignment E=3.2e-18 PF18267: Rubredoxin_C" amino acids 315 to 384 (70 residues), 38.4 bits, see alignment E=3e-13 PF04324: Fer2_BFD" amino acids 419 to 466 (48 residues), 34.3 bits, see alignment 7.1e-12 PF03460: NIR_SIR_ferr" amino acids 554 to 617 (64 residues), 41.9 bits, see alignment 2.1e-14 PF01077: NIR_SIR" amino acids 627 to 748 (122 residues), 73.8 bits, see alignment E=3.2e-24

Best Hits

Swiss-Prot: 62% identical to NIRB_ECOLI: Nitrite reductase (NADH) large subunit (nirB) from Escherichia coli (strain K12)

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 100% identity to bpy:Bphyt_1983)

MetaCyc: 62% identical to nitrite reductase (NADH) large subunit (Escherichia coli K-12 substr. MG1655)
RXN-13854 [EC: 1.7.1.15]

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.15 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T479 at UniProt or InterPro

Protein Sequence (846 amino acids)

>BPHYT_RS09840 nitrite reductase (Burkholderia phytofirmans PsJN)
MKIIVIGHGMVGHKLVECLAQDAAHGLDITVLCEESRPAYDRVHLSEFFAGKTADDLSLV
EPGFFERQNVLLKLNAKAVAIDRDARTVTVSTGETLPYDKLVFATGSYPFVPPVQGRERA
DCFVYRTIDDLEAMRECGARSKTGTVVGGGLLGLECAKALRDMGLQTHVVEFAPRLMAVQ
VDDGGGRVLRGKIEELGVTVHMQKNTTAIVDGEAGTHRMQFADGSHLDTDMIVFSAGIRP
RDDLARASGLTLGPRGGIVIDNACRTSDPHIYAIGECALWNGQLFGLVAPGYDMARAVAK
QLLGDTAEFAGADMSTKLKLMGVDVASIGDAHGNTPGSRAYQFSDERKQVYKKLVVSECG
KYLLGGVMVGDAAEYGTLLQMMLNRIELPASPEFLILPAADGNAKPALGVDSLPASAQIC
SCNNVSKGELCAAVCAGATDIGALKCATTAGTSCGGCVPLVTQVMKAEMKKQGLAVNNHV
CEHFAYSRQELYHIVRVEGVRSFGELLAKHGHGLGCDICKPVVASVLASCWNEFVLKKEH
ASLQDTNDYYLANIQRDGTYSVVPRMAGGEVTPDGLIAVGQVAKKYGLYTKITGGQRVDL
FGARVEQLPLIWEELIAAGFESGHAYGKSLRTVKSCVGSTWCRYGVGDSVGLAVEIENRY
KGLRTPHKIKFGVSGCTRECAEAQGKDVGIIATEKGWNLYVCGNGGMKPRHAELLASDLD
KETLVRYIDRFLMFYVRTADRLQRTSVWRENLEGGLDYLIDVVVNDHLGVADELESEMQH
VVDTYECEWKKAVTDPETRKRFRHFVNSGESDSNVAFVEERGQIRPATPAERRSKLAAIP
VVVETV