Protein Info for BPHYT_RS09460 in Burkholderia phytofirmans PsJN

Annotation: peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 807 PF02190: LON_substr_bdg" amino acids 13 to 203 (191 residues), 203.4 bits, see alignment E=9.9e-64 TIGR00763: endopeptidase La" amino acids 15 to 773 (759 residues), 992.8 bits, see alignment E=7.4e-303 PF00004: AAA" amino acids 354 to 492 (139 residues), 75.1 bits, see alignment E=1.8e-24 PF07728: AAA_5" amino acids 354 to 487 (134 residues), 38.6 bits, see alignment E=2.6e-13 PF05362: Lon_C" amino acids 572 to 774 (203 residues), 323.1 bits, see alignment E=1.7e-100 PF13541: ChlI" amino acids 615 to 742 (128 residues), 32.3 bits, see alignment E=2.1e-11

Best Hits

Swiss-Prot: 70% identical to LON_ECOLI: Lon protease (lon) from Escherichia coli (strain K12)

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 96% identity to bph:Bphy_1013)

MetaCyc: 70% identical to Lon protease (Escherichia coli K-12 substr. MG1655)
Endopeptidase La. [EC: 3.4.21.53]

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.53

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T405 at UniProt or InterPro

Protein Sequence (807 amino acids)

>BPHYT_RS09460 peptidase (Burkholderia phytofirmans PsJN)
MSGTQLLPPERITLPLLPLRDVVVFPHMVIPLFVGRPKSIKALEAAMEGGKHIMLVAQKT
AAKDEPTEKDMYEVGCVANILQMLKLPDGTVKVLVEGLQRAKTLSIEEQETQFSCEVMPL
EPDHADSAETEALRRAIVSQFDQYVKLNKKIPPEILTSLSGIDEAGRLADTIAAHLPLKL
DQKQHILEMFPVIERLEHLLAQLEAEIDILQVEKRIRGRVKRQMEKSQREYYLNEQVKAI
QKELGEGEEGADLEELEKRITAARMPKEAKKKADAELKKLKLMSPMSAEATVVRNYIDTL
IGLPWRKKSKVNNDLSNAERVLDEDHFGLEKVKERILEYLAVQQRVDKVKAPILCLVGPP
GVGKTSLGQSIARATNRKFVRMALGGVRDEAEIRGHRRTYIGSMPGKILQSLTKVGVRNP
LFLLDEVDKMGQDFRGDPSSALLEVLDPEQNHTFADHYVEVDFDLSDVMFVATSNSLNIP
PPLLDRMEVIRLSGYTEDEKVSIAQRYLLPKQKKNNGLKDGEVDVTETAIRDIIRYYTRE
AGVRSLEREISKICRKVVKMLLLKKAEGAVKVDGSNLDTFLGVRKYDFGLAAKENQVGQV
TGLAWTEVGGDLLTIEAAVMPGKGNVIRTGSLGDVMKESVEAARSVVRSRSRRLGVKDEA
FEKQDIHIHVPEGATPKDGPSAGIAMTTALVSVLTGIPVRADVAMTGEITLRGEVLPIGG
LKEKLLAAHRGGIKLVLIPEENVKDLTEIPDNVKNAIEIVPVRWIDKVLELALERVPTAL
PEEEPKPAAPVAAEPGKDAAATEVVKH