Protein Info for BPHYT_RS09280 in Burkholderia phytofirmans PsJN
Annotation: pyridoxal-5'-phosphate-dependent protein subunit beta
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 67% identical to FSDH_RALPI: Phenylserine dehydratase (psdht) from Ralstonia pickettii
KEGG orthology group: K01754, threonine dehydratase [EC: 4.3.1.19] (inferred from 100% identity to bpy:Bphyt_1872)MetaCyc: 35% identical to L-threo-3-hydroxy aspartate ammonia-lyase (Pseudomonas sp. T62)
Threo-3-hydroxyaspartate ammonia-lyase. [EC: 4.3.1.16]
Predicted SEED Role
"Putative amino-acid dehydratase (EC 4.2.-.-)" (EC 4.2.-.-)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- superpathway of L-threonine metabolism (16/18 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- L-threonine degradation I (5/6 steps found)
- L-methionine degradation II (2/3 steps found)
- L-threonine degradation V (1/2 steps found)
- hypoglycin biosynthesis (4/14 steps found)
- cyclosporin A biosynthesis (2/15 steps found)
KEGG Metabolic Maps
- Aminosugars metabolism
- Glycine, serine and threonine metabolism
- Nucleotide sugars metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 4.2.-.-, 4.3.1.19
Use Curated BLAST to search for 4.2.-.- or 4.3.1.16 or 4.3.1.19
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2T3W9 at UniProt or InterPro
Protein Sequence (344 amino acids)
>BPHYT_RS09280 pyridoxal-5'-phosphate-dependent protein subunit beta (Burkholderia phytofirmans PsJN) MSTATPQHTDHTIDGEPIPTLDDIAAQHFALTPWVARTPVFDRVDFPSLEGTVVNFKFEL LQAGGSFKARGAFTNLLALDEAQRSAGVTCVSGGNHAVAVAYAAMRLGVSAKVVLFRAAN PARVALCRQYRAEIVFAEDIAEAFELVRRIEAEEGRYFVHPFNGYRTVLGSATLGYEWAT QTPDLEAVILPIGGGGLAAGVATAMRLANPNVHVYGVEPEGADVMGKSFAANHTVKMRQM RGIADSLMSPHTEQYSYELCRRHIDQLVTVSDDQLRAAMLTLFGQLKLAVEPACAAATAG LLGPLREQLQGKRVGVLLCGTNTDPVTFAAHIERARHSESQFPQ