Protein Info for BPHYT_RS08875 in Burkholderia phytofirmans PsJN

Annotation: DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 TIGR00665: replicative DNA helicase" amino acids 16 to 452 (437 residues), 579.9 bits, see alignment E=1.6e-178 PF00772: DnaB" amino acids 16 to 117 (102 residues), 122.3 bits, see alignment E=1.6e-39 PF03796: DnaB_C" amino acids 196 to 451 (256 residues), 366 bits, see alignment E=2e-113 PF13481: AAA_25" amino acids 212 to 374 (163 residues), 42.1 bits, see alignment E=1.5e-14

Best Hits

Swiss-Prot: 56% identical to DNAB_SALTY: Replicative DNA helicase (dnaB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 100% identity to bxe:Bxe_A2452)

MetaCyc: 56% identical to replicative DNA helicase (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12 or 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T3N7 at UniProt or InterPro

Protein Sequence (461 amino acids)

>BPHYT_RS08875 DNA helicase (Burkholderia phytofirmans PsJN)
MNAPSKDPQLESLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFLSQSDFYRYDHRIIFEH
IGKLIAATRPADVITVYEALGTSGKAEEVGGLAYLNALAQNTPSAANIRRYAEIVRDRAV
LRRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAEDGARGTQGFLEIGPLLTEVVE
RIDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAV
EYGLPVAVFSMEMPGSQLTMRMLGSVGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQIF
IDETGGLNPMELRSRARRLSRQCGKLGLIIIDYLQLMSGSSSGENRATEISEISRSLKSL
AKELDVPVIALSQLNRGLEQRPNKRPIMSDLRESGAIEQDADVILFIYRDEVYNPDSPDK
GTAEIIIGKQRNGPIGPVRLTFHGQFTKFDNFAGAQNFYSD