Protein Info for BPHYT_RS08830 in Burkholderia phytofirmans PsJN

Annotation: hydroxypyruvate isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03234: hydroxypyruvate isomerase
  • amino acids 2 to 254 (253 residues), 384.3 bits, E=1.2e-119
• PF01261: AP_endonuc_2
  • amino acids 21 to 255 (235 residues), 169.3 bits, E=6.6e-54

Best Hits

Swiss-Prot: 56% identical to HYI_ECOLI: Hydroxypyruvate isomerase (hyi) from Escherichia coli (strain K12)

KEGG orthology group: K01816, hydroxypyruvate isomerase [EC: 5.3.1.22] (inferred from 100% identity to bpy:Bphyt_1781)

MetaCyc: 56% identical to hydroxypyruvate isomerase (Escherichia coli K-12 substr. MG1655)
Hydroxypyruvate isomerase. [EC: 5.3.1.22]

Predicted SEED Role

"Hydroxypyruvate isomerase (EC 5.3.1.22)" (EC 5.3.1.22)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.22

Use Curated BLAST to search for 5.3.1.22

Sequence Analysis Tools

See B2T3M8 at UniProt or InterPro

Protein Sequence (269 amino acids)

>BPHYT_RS08830 hydroxypyruvate isomerase (Burkholderia phytofirmans PsJN)
MPKFAANLTMLFNEVPFLDRFAAAADAGFHAVEFLFPYPYQIAELSERLQQNRLKLVLHN
LPAGNWEAGERGIACLPDRVSEFQEGVGRAIEYAKALKVPQLNCLVGIPTAGVDADKARS
TIVDNLRFAASELKKAGIKLLVEPCNSYDIPGFALNHSGEGLDVIRAVGSDNLYLQYDIY
HMQRMEGELAATIRKNLPQIAHIQLADNPGRNEPGTGEINYPFLFDLLDTLGYDGYVGCE
YKPRTTTTAGLGWVQSVAGQTRGAAHAAA