Protein Info for BPHYT_RS07975 in Burkholderia phytofirmans PsJN

Annotation: exodeoxyribonuclease V subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1232 TIGR00609: exodeoxyribonuclease V, beta subunit" amino acids 16 to 1225 (1210 residues), 831.6 bits, see alignment E=6.8e-254 PF00580: UvrD-helicase" amino acids 20 to 446 (427 residues), 173.1 bits, see alignment E=2.5e-54 PF13361: UvrD_C" amino acids 691 to 843 (153 residues), 43.5 bits, see alignment E=7.5e-15 PF12705: PDDEXK_1" amino acids 1001 to 1174 (174 residues), 25.1 bits, see alignment E=4.1e-09

Best Hits

KEGG orthology group: K03582, exodeoxyribonuclease V beta subunit [EC: 3.1.11.5] (inferred from 70% identity to bcj:BCAL1097)

Predicted SEED Role

"Exodeoxyribonuclease V beta chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T360 at UniProt or InterPro

Protein Sequence (1232 amino acids)

>BPHYT_RS07975 exodeoxyribonuclease V subunit beta (Burkholderia phytofirmans PsJN)
MSGAMKHHALVAEELDVFACSLDGVNQIEASAGTGKTWNICALYVRLLLEKNLNADQILV
VTFTKAATAELHERIRGRLAELDRAIDMDDDGGDPFIRRLFETTLAPERGIAREDALKVV
RRALRTFDQAAIHTIHAFCQRALQEAPFAAAMPFAFEMEADDAALRFEMAADFWREQVEP
VAHAHPAFAAWLVAKRAGPASLDEQLARRLKKPLAQLRWGKLDANGTGADPQAGFDAACE
VWHAERDAIVRLLAEAQDRLSKTSHKPDHVSAAIAAWTEYFAQGDCHAPPPKAALKLTAS
ALKKATKVKFEPPEHAFFEHAEALAAAVVAAEAAQRARWLSLVQTWLDYAPAELVARKRT
RRVVSFDDLLSNLYRALAAHPWLADALRTRYPAALIDEFQDTDPLQFAIFSRIFAPAGPL
FLVGDPKQAIYSFRAADLHTYLAAREAASACYTLAVNQRSTAPIVEACNRLFEANQQAFV
LEGLDYQPVRAGERRRPPFSDPDASAGDFRIWTLPQGEAALTKREAQREASEACAAEIVR
LLRGAREGTVTIGDEPLAPGNIAVLVQTHKQGSLIKRVLAAWGVGSVELAQASVFATLDA
EQIERVLAAVDTPGDLRRLRAALATDWLGLDAAALWRLEQVADAPSVADDPAHAADAMGW
VERFSRYRTLWHERGFAVMWRTLMRELRVAQRLVAAADGERRLTNVNHLAELVQARAATQ
PGIAPTLRWLAAQRTQGGGEDAQLRLESDRNLVQIVTVHKSKGLEYAVVFCPFLNDGGLR
EPPSSGLPDAREYHDETGDAVLHYGCDDEEAEHASRYAVREQAAERARLVYVALTRAVYR
CYLVAGTYQSSRSTKESRRSVLNWLVAGEGHDFGDWLAEPPDEAALSARWQALAGGPVTL
AALPKPARRVPLESLLDRSARMQARANRRPLRDQWRMASFSGLIAAGSKSEETHAPVEEV
RPDHDEIADALAVTSTLAPAPQAPVYAEDDILAFPRGAAAGDCLHRMFELADFSEPHTWT
DAIRGALRERPAPAVSELAAHLPAMMHNLLRDVVTAELQPGMTLARLNPRRRLNELEFLF
AAPSLDFPALRELLIEHGYPDVALEPGVLRGFVKGFIDMIVEYDGRFWIVDWKSNHLGDT
AADYAAAPLEAAMASHAYHLQALLYTVALHRYLRTRVRDYSYDTHIGGYLYLFVRGVRPH
WRDADGPAGVHMRRPAFELVALLDAAMIGGVA