Protein Info for BPHYT_RS07010 in Burkholderia phytofirmans PsJN

Annotation: sodium:solute symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 672 transmembrane" amino acids 7 to 26 (20 residues), see Phobius details amino acids 37 to 56 (20 residues), see Phobius details amino acids 77 to 100 (24 residues), see Phobius details amino acids 106 to 127 (22 residues), see Phobius details amino acids 147 to 166 (20 residues), see Phobius details amino acids 178 to 200 (23 residues), see Phobius details amino acids 211 to 230 (20 residues), see Phobius details amino acids 368 to 389 (22 residues), see Phobius details amino acids 402 to 423 (22 residues), see Phobius details amino acids 491 to 518 (28 residues), see Phobius details amino acids 538 to 556 (19 residues), see Phobius details amino acids 562 to 586 (25 residues), see Phobius details amino acids 593 to 614 (22 residues), see Phobius details amino acids 634 to 654 (21 residues), see Phobius details TIGR03648: probable sodium:solute symporter, VC_2705 subfamily" amino acids 40 to 672 (633 residues), 727 bits, see alignment E=1.3e-222 PF00474: SSF" amino acids 65 to 230 (166 residues), 119.6 bits, see alignment E=8e-39 amino acids 487 to 602 (116 residues), 66.8 bits, see alignment E=8.7e-23

Best Hits

Swiss-Prot: 63% identical to Y2524_CUPNH: Uncharacterized symporter H16_A2524 (H16_A2524) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K14393, cation/acetate symporter (inferred from 100% identity to bpy:Bphyt_1418)

Predicted SEED Role

"Acetate permease ActP (cation/acetate symporter)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T2M2 at UniProt or InterPro

Protein Sequence (672 amino acids)

>BPHYT_RS07010 sodium:solute symporter (Burkholderia phytofirmans PsJN)
MKLTNRLISSYALYTLGFLLFIYVMWRIERTTGPGVWIGYVFLFVPIAVYAVIGLLSRTS
DLVEYYVAGRRVPSAFNGMATAADWLSAASFIGLAGSIYATGYDGLAYLMGWTGGYCLVA
FLLAPYVRKLARYTIPDFLGTRFSSNAVRGLAALAAVLCSFVYLVAQIQGVGLIATRFIG
VDFAVGIFCGLAGILVCSFLGGMRAVTWTQVAQYIILIAAILIPVSMIAHKDGLGWVPQF
NYGRLMERVEGLEKQVRDDPLEQTVRDDYRRRAALMQMRLDTLPQSFVDEKLRLTQEVAD
LRRHNGPLREIKERERSLEQFPRDAAAAQIVWAQRRDEMLMRAAAPVPMHEPFPAVSDED
RRIHERNFLSLLLCLSLGTASLPHILTRYNTTTSVASARRSVGWTLFFVALFYLTVPVLA
VLIKYEILTNLVGHHFSDLPQWLMQWRKVEPSLISLADTNGDGIVRWSEIQMQPDMVVLA
APEIAGLPYVMSGLIAAGALAAALSTADGLLLTIANALSHDVYYHMVDPNASSQRRVTIS
KILLLGVALFASYVASLNTGNILFLVGAAFSLAASSLFPVLVLGVFWKRTTRLGAVAGMV
AGLVVCIYYIVSTYPFFTQMTGFAGPRWFGIEPISSGVFGVPAGFLVAIGVSLIDRKPDA
YTRALVDYIRHP