Protein Info for BPHYT_RS05240 in Burkholderia phytofirmans PsJN

Annotation: heme biosynthesis protein HemY

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 44 to 64 (21 residues), see Phobius details TIGR00540: heme biosynthesis-associated TPR protein" amino acids 4 to 389 (386 residues), 423.6 bits, see alignment E=3.1e-131 PF07219: HemY_N" amino acids 28 to 133 (106 residues), 101.9 bits, see alignment E=4.3e-33 PF13424: TPR_12" amino acids 329 to 389 (61 residues), 31.4 bits, see alignment E=3.5e-11

Best Hits

KEGG orthology group: K02498, HemY protein (inferred from 100% identity to bpy:Bphyt_1071)

Predicted SEED Role

"Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T1D3 at UniProt or InterPro

Protein Sequence (396 amino acids)

>BPHYT_RS05240 heme biosynthesis protein HemY (Burkholderia phytofirmans PsJN)
MAIRGLLWLALLFVIAVVLAVVGRFDMGQVLLIYPPYRVDISLNLFVVGLVVLFILIYAL
LRIFRNIWRMPQRVAAYRARSRVAKAHAALRDAIGNLYAGRFSRAEKAAKDALANGDNKG
AAGLIAATAAHRMHEYARRDEWLSQIDEADWQDARLMATADMRADGRDADGALTALTQMQ
SQGGRRIHAQQIMLRAQQQLKNWGEVLKLVKTLEKREAIHPAVAVRLRQLAAENLLRDRR
HNADALLELWNSLSATERHSPRLADLAAELLVALNRPQEARKIVEEALAQNWDARLLRRY
PDTAGGDALPLIQKAEGWQKDRPEDADLMFALGRLCLHQQLWGKAQSFLERALKLADNET
LKIRSHRALARLHEQLGDAEKASQHYRESALAMNIV