Protein Info for BPHYT_RS05200 in Burkholderia phytofirmans PsJN

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 transmembrane" amino acids 24 to 52 (29 residues), see Phobius details amino acids 61 to 83 (23 residues), see Phobius details amino acids 95 to 116 (22 residues), see Phobius details amino acids 122 to 148 (27 residues), see Phobius details amino acids 162 to 185 (24 residues), see Phobius details amino acids 196 to 216 (21 residues), see Phobius details amino acids 239 to 264 (26 residues), see Phobius details amino acids 284 to 306 (23 residues), see Phobius details amino acids 315 to 335 (21 residues), see Phobius details amino acids 341 to 364 (24 residues), see Phobius details amino acids 376 to 396 (21 residues), see Phobius details amino acids 407 to 426 (20 residues), see Phobius details PF00083: Sugar_tr" amino acids 23 to 234 (212 residues), 95.8 bits, see alignment E=2.8e-31 amino acids 261 to 433 (173 residues), 40.4 bits, see alignment E=1.8e-14 PF07690: MFS_1" amino acids 27 to 378 (352 residues), 92.1 bits, see alignment E=3.5e-30 amino acids 284 to 426 (143 residues), 46.3 bits, see alignment E=2.9e-16

Best Hits

Swiss-Prot: 54% identical to YHJE_ECOLI: Inner membrane metabolite transport protein YhjE (yhjE) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_1063)

MetaCyc: 31% identical to osmolyte:H+ symporter ProP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-29; TRANS-RXN-29A

Predicted SEED Role

"Permeases of the major facilitator superfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T1C5 at UniProt or InterPro

Protein Sequence (435 amino acids)

>BPHYT_RS05200 MFS transporter (Burkholderia phytofirmans PsJN)
MSTSPISAAQPNAAGQNKSSRIIFASFIGTAIEFYDFYVYATAAALVIGPVFFPHGSATA
QALSAFVTFGIAFVARPIGSFLFGHFGDRIGRKSTLVASLLVMGVSTTLIGFVPGYDSIG
SLAPILLCILRFGQGIGLGGEWGGAALLATENAPAGKRGWFGMFPQLGPSIGFLASNGLF
FALALSLSDEQFRSWGWRVPFLVSAVLVALGLYVRLKIAETPAFQAAIERQERVKVPIAT
LLSQHWVPTLLGALAMVVCYTLFYNATTFSLSYGVSVLHIPRPTFLGLLCIAVVFMALAT
PLSAWAADRYGRKPVLIVGIIAAILSGFTMAPLLGSGQTSLVLLFLVIELFLMGVTFAPM
GALLPELFPTNVRYTGAGVSYNLGGILGASVAPYIAQMLAARGGLPWVGAYVSIAAAVSM
IGVLCMRETRDTQLM