Protein Info for BPHYT_RS03800 in Burkholderia phytofirmans PsJN

Annotation: homogentisate 1,2-dioxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 432 TIGR01015: homogentisate 1,2-dioxygenase" amino acids 4 to 427 (424 residues), 665.6 bits, see alignment E=1.4e-204 PF20510: HgmA_N" amino acids 5 to 273 (269 residues), 413.7 bits, see alignment E=3.5e-128 PF04209: HgmA_C" amino acids 274 to 427 (154 residues), 245.1 bits, see alignment E=2.4e-77

Best Hits

Swiss-Prot: 87% identical to HGD_BURM9: Homogentisate 1,2-dioxygenase (hmgA) from Burkholderia mallei (strain NCTC 10229)

KEGG orthology group: K00451, homogentisate 1,2-dioxygenase [EC: 1.13.11.5] (inferred from 100% identity to bpy:Bphyt_0780)

MetaCyc: 52% identical to Homogentisate 1,2-dioxygenase (Homo sapiens)
Homogentisate 1,2-dioxygenase. [EC: 1.13.11.5]

Predicted SEED Role

"Homogentisate 1,2-dioxygenase (EC 1.13.11.5)" in subsystem Homogentisate pathway of aromatic compound degradation (EC 1.13.11.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T098 at UniProt or InterPro

Protein Sequence (432 amino acids)

>BPHYT_RS03800 homogentisate 1,2-dioxygenase (Burkholderia phytofirmans PsJN)
MEPGYQSGFANEFATEALPGALPQGRNSPQRAAYGLYAEQLSGTAFTAPRGHNRRSWLYR
IRPAAVHMPFTPLPSERLVANFAEVPPTPPNQLRWDALPMPAEPTDFIDGWVTMAGNGAA
ESMTGCAIHVYAANRSMQDRFFYTADGELLIVPQEGRLHIATEFGKLDVGPFEIAVIPRG
VRFAVSLPDGVARGYICENFGALLRLPDLGPIGSNGLANPRDFLTPHAAYEDREGDFELV
AKMNGNLWRADIGHSPLDVVAWHGNYAPYKYDLRRFNTIGSISFDHPDPSIFLVLQSQTD
TPGVDSIDFVIFPPRWLAAEDTFRPPWFHRNVASEFMGLVHGVYDAKAEGFVPGGASLHN
CMSGHGPDAETFEKASHSDTSTPKKVGDTMAFMFETRTLIKPTRFALETAQLQAHYYECW
QGLTKHFNPEQR