Protein Info for BPHYT_RS03590 in Burkholderia phytofirmans PsJN

Annotation: chaperone protein DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 TIGR02350: chaperone protein DnaK" amino acids 3 to 606 (604 residues), 949.3 bits, see alignment E=3.7e-290 PF00012: HSP70" amino acids 4 to 606 (603 residues), 879.2 bits, see alignment E=1.3e-268 PF06723: MreB_Mbl" amino acids 116 to 378 (263 residues), 42.5 bits, see alignment E=3.8e-15

Best Hits

Swiss-Prot: 100% identical to DNAK_PARPJ: Chaperone protein DnaK (dnaK) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 100% identity to bpy:Bphyt_0737)

MetaCyc: 72% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SXC6 at UniProt or InterPro

Protein Sequence (650 amino acids)

>BPHYT_RS03590 chaperone protein DnaK (Burkholderia phytofirmans PsJN)
MGKIIGIDLGTTNSCVAIMEGNSVKVIENSEGARTTPSIIAYMEDGEILVGAPAKRQSVT
NPKNTLYAVKRLIGRRFEEKEVQKDIALMPYKIMKADNGDAWIEVRDQKLAPPQISAETL
RKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRIAGLEVKRIINEPTAAALAFG
LDKAEKGDRKIAVYDLGGGTFDVSIIEIADVDGEMQFEVLSTNGDTFLGGEDFDQRIIDY
IIAEFKKEQGVDLSKDVLALQRLKESAEKAKIELSSSQQTEINLPYITADASGPKHLDLK
ITRAKLEALVEELIERTIEPCRVAIKDAGVKVGEIDDVILVGGMTRMPKVQEKVKEFFGK
DPRRDVNPDEAVAVGAAIQGQVLSGDRKDVLLLDVTPLSLGIETLGGVMTKMINKNTTIP
TKHAQVYSTADDNQGAVTIKVFQGEREMAAGNKLLGEFNLEGIPPAPRGTPQIEVSFDID
ANGILHVGAKDKATGKENRITIKANSGLSEAEIEKMVKDAEANAEEDHKLRELADARNQG
DALVHSTKKALTEYGDKLEAAEKEKIEAALKDLEETLKSGSADKAAIEAKIEVVATASQK
MGEKMYADMQAAQGAEAAAAGAAGAGATAGGASQQQDDVVDAEFKEVKKD