Protein Info for BPHYT_RS03150 in Burkholderia phytofirmans PsJN

Updated annotation (from data): Phosphoglycerate dehydrogenase (EC:1.1.1.95)
Rationale: Auxotrophic, and rescued by L-serine or gly-glu. Glycine could yield serine via hydroxymethyltransferase, so this is consistent with a role in serine synthesis. BPHYT_RS31960 is also a possibility but gene context suggests that has a role in catabolism and has little phenotype; BPHYT_RS01425 is a possibility, but fitness data suggests it is cell wall related (auxotroph)
Original annotation: FAD-linked oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF12447: DUF3683
  • amino acids 23 to 147 (125 residues), 176.4 bits, E=5.8e-56
• PF01565: FAD_binding_4
  • amino acids 197 to 342 (146 residues), 80.9 bits, E=2.3e-26
• PF02913: FAD-oxidase_C
  • amino acids 461 to 579 (119 residues), 29 bits, E=2.6e-10
  • amino acids 730 to 818 (89 residues), 65.8 bits, 1.5e-21
• PF13183: Fer4_8
  • amino acids 854 to 933 (80 residues), 33.7 bits, 1.3e-11
• PF02754: CCG
  • amino acids 1064 to 1153 (90 residues), 30 bits, 1.4e-10
  • amino acids 1190 to 1255 (66 residues), 17.3 bits, 1.3e-06
• PF11880: DUF3400
  • amino acids 1320 to 1364 (45 residues), 86.5 bits, see alignment (E = 2.1e-28)

Predicted SEED Role

"FAD/FMN-containing dehydrogenases"

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• L-serine biosynthesis I (3/3 steps found)
• L-cysteine biosynthesis IX (Trichomonas vaginalis) (2/3 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.95

Use Curated BLAST to search for 1.1.1.95

Sequence Analysis Tools

See B2SX32 at UniProt or InterPro

Protein Sequence (1364 amino acids)

>BPHYT_RS03150 Phosphoglycerate dehydrogenase (EC:1.1.1.95) (Burkholderia phytofirmans PsJN)
MNAPQVFDPHGAAATVAADPEARLREIPYNYTSFSDREIVIRLLGDEAWAVLAELRAERR
TGRSARMLYEVLGDIWVVRRNPYLQDDLLDNPKRRAMLIEALHHRLSEIEKRRRADLTEH
GDEAGVDRAARVETLVQAARRAVDEFASEFQKTYDLRKRATKVLGKVTEKDNIKFDGLSR
VSHVTDATDWRVEYPFVVLTPDTEAEIAGMIKACFELGLTVIPRGGGTGYTGGAVPLTPF
SAVINTEKLEQLGAVEMTELPGVDRKVATIFSGAGVVTRRVTEAAEQAGFVFAVDPTSLD
ASCVGGNVAMNAGGKKAVLWGTALDNLAWWRMVDPEGNWLEVTRLDHNMGKIHDIEVARF
RLDWFDGNYAPGEKLMRTEALDIKGRVFRKEGLGKDVTDKFLAGLPGVQKEGCDGLITSA
RWVLHKMPAHTRTVCLEFFGQARDAIPSIVEIKDYLFETSKQGGAILAGLEHLDERYLRA
VGYATKSKRNAFPKMVLIGDIVGDDADAVAQATSEVVRMANGKSGEGFVAVNAEARKRFW
LDRSRTAAIAKHTNAFKINEDVVIPLDRMGEYTDGIERINIELSIKNKLQLVDALEAFFK
GGKLPLGKSDDANEIPSAELLEDRVQQALDLLKRVRTRWEFLRDKLDLSLREAQHYLVGL
GYEAMAEKFADRVDAQPDVNVFHVTQDRTIRVSWKQEIRAELRQIFNGGEFKPILDEAQA
IHKQVLRGRVFVALHMHAGDGNVHTNLPVNSDNYEMLQDAHTAVARIMKLARSLDGVISG
EHGIGITKLEFLTEDEISEFRQYKQRVDPHGRFNAGKLLEGADLRNAYTPSFGLMGYESL
IMQQSDIGAISESIKDCLRCGKCKPVCATHVPRANLLYSPRNKILATSLLVEAFLYEEQT
RRGVSIKHWDEFNDVADHCTVCHKCVTPCPVKIDFGDVTMNMRNLLRKMGKKKFNPGNAA
GMFFLNATNPQTINLARTAMMGVGYKAQRLGNEVLKKFTKKQTAHPPATVGKPPVTQQVI
HFMNKKMPGNLPKKTARALLDIEDNKIVPIIRNPKTTTADTEAVFYFPGCGSERLFSQVG
LATQAMLWEAGVQTVLPPGYLCCGYPQRGSGQFDKAEQIVTDNRVLFHRVANTLNYLDIK
TVVVSCGTCYDQLAGYEFEKIFPGCRIIDIHEFLLEKGMKLDGVNGVRYMYHDPCHTPIK
TMDPIKLVNQLMGSEKDGYKIEKNDRCCGESGTLAVTRPDISTQVRFRKEEEIRKGAAKL
RGIPLVAEAGANGINPANASAGSAGAPEGSVLKAGDGPQPKGATDVKILTSCPSCLQGLS
RYNEDAGIEADYIVVEMARHVLGEDWMVDYVQRANNGGIERVLV