Protein Info for BPHYT_RS01995 in Burkholderia phytofirmans PsJN

Annotation: pyrimidine permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 transmembrane" amino acids 32 to 54 (23 residues), see Phobius details amino acids 60 to 76 (17 residues), see Phobius details amino acids 83 to 102 (20 residues), see Phobius details amino acids 109 to 133 (25 residues), see Phobius details amino acids 141 to 162 (22 residues), see Phobius details amino acids 173 to 190 (18 residues), see Phobius details amino acids 197 to 217 (21 residues), see Phobius details amino acids 241 to 261 (21 residues), see Phobius details amino acids 282 to 306 (25 residues), see Phobius details amino acids 319 to 338 (20 residues), see Phobius details amino acids 350 to 373 (24 residues), see Phobius details amino acids 405 to 425 (21 residues), see Phobius details TIGR00801: uracil-xanthine permease" amino acids 28 to 423 (396 residues), 356.2 bits, see alignment E=1.2e-110 PF00860: Xan_ur_permease" amino acids 31 to 399 (369 residues), 309.8 bits, see alignment E=1.1e-96

Best Hits

Swiss-Prot: 71% identical to RUTG_ECOLI: Putative pyrimidine permease RutG (rutG) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_0413)

MetaCyc: 71% identical to pyrimidine:H+ symporter (Escherichia coli K-12 substr. MG1655)
RXN-5076; TRANS-RXN-132; TRANS-RXN-362

Predicted SEED Role

"Xanthine permease" in subsystem Purine Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SWP4 at UniProt or InterPro

Protein Sequence (435 amino acids)

>BPHYT_RS01995 pyrimidine permease (Burkholderia phytofirmans PsJN)
MADSYFPRWRPQSAAAEGRIVGTDERLAWPQMFAMGVQHVVAMFGSTVLAPLLMGFDPNL
CIFMSGIGTLLFFVLVGGRVPSYLGSSFAFIGLVIAVTGYGGHGPNLNIPVALGGIIACG
VVYAIIGLIVSAVGTRWIETLIPPVVTGAIVCVIGLNLAPIAVHGVSGSNFDSWMALVTV
LCVGAVAVFARGMLQRLLILVGLLMAYVIYAIVTNGLGMGKPIDFAIVANAAWFGMPHFT
APVFNMQAMTLLAPIAVILVAENLGHIKAVSAMTGQNLDRYVGRAFLGDGLATIVSGFAG
GTGVTTYAENIGVMAVTKIYSTLVFVIAALIALVLGFSPKFGAVIQTIPGPVLGGVSIVV
FGLIAVTGARIWVVNKVDFSDNRNLIVAAVTLVLGAGDFSLKLGGFGLGGIGTATFGAII
LYALLRRKPAQGLVA