Protein Info for BPHYT_RS01940 in Burkholderia phytofirmans PsJN

Annotation: 16S rRNA methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 293 PF00590: TP_methylase" amino acids 18 to 216 (199 residues), 105.4 bits, see alignment E=2e-34 TIGR00096: 16S rRNA (cytidine(1402)-2'-O)-methyltransferase" amino acids 18 to 285 (268 residues), 246.2 bits, see alignment E=2.1e-77

Best Hits

Swiss-Prot: 52% identical to RSMI_XYLFT: Ribosomal RNA small subunit methyltransferase I (rsmI) from Xylella fastidiosa (strain Temecula1 / ATCC 700964)

KEGG orthology group: K07056, (no description) (inferred from 100% identity to bpy:Bphyt_0404)

Predicted SEED Role

"rRNA small subunit methyltransferase I" in subsystem Heat shock dnaK gene cluster extended

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SWN5 at UniProt or InterPro

Protein Sequence (293 amino acids)

>BPHYT_RS01940 16S rRNA methyltransferase (Burkholderia phytofirmans PsJN)
MTPLSELAQGQQYPTAALYVVATPIGNIADVTLRALHVLGLVDRIAAEDTRNTGQLLARY
GISKPLVAVHQHNERAAALRLIEHLQAGERVAYVSDAGTPGISDPGAKLVDAVREAGFPV
IPLPGASALATALSVAGDWVATFSFLGFLPPKAKARAATLQPLATHPHAMVFYEAPHRIV
ETVQALADAFGGERKLLIARELTKLHEALHRGTLAEGPTWLAADPNRQRGEFVLVVEGAQ
PETAGEHDHDALLGILLEELTVSSAAKVAAAITGASRNALYTRALTLKKEDEE