Protein Info for BPHYT_RS01570 in Burkholderia phytofirmans PsJN

Annotation: exodeoxyribonuclease III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 259 TIGR00633: exodeoxyribonuclease III (xth)" amino acids 2 to 253 (252 residues), 256.4 bits, see alignment E=2.9e-80 TIGR00195: exodeoxyribonuclease III" amino acids 3 to 251 (249 residues), 241 bits, see alignment E=1.6e-75 PF03372: Exo_endo_phos" amino acids 5 to 246 (242 residues), 97.3 bits, see alignment E=5.8e-32

Best Hits

Swiss-Prot: 37% identical to EXOA_BACSU: Exodeoxyribonuclease (exoA) from Bacillus subtilis (strain 168)

KEGG orthology group: K01142, exodeoxyribonuclease III [EC: 3.1.11.2] (inferred from 100% identity to bpy:Bphyt_0331)

Predicted SEED Role

"Exodeoxyribonuclease III (EC 3.1.11.2)" in subsystem DNA repair, bacterial (EC 3.1.11.2)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.2

Use Curated BLAST to search for 3.1.11.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T1M7 at UniProt or InterPro

Protein Sequence (259 amino acids)

>BPHYT_RS01570 exodeoxyribonuclease III (Burkholderia phytofirmans PsJN)
MLRVITANLNGIRSAAKKGFFDWFGEQKADVLCVQEIKCSQDDMTPEFMAPHNFTGYFQH
AVKKGYSGAGVYTRHEPDEVVIGFGSEEFDPEGRYVELRFGKLSVISVYVPSGSSGDERQ
QAKYRFMDEFMPHLAELSQEREVIVCGDVNIVHKEIDIKNWKSNQKNSGCLPEERAWLTK
LFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIASKAKRTDVFRD
IKFSDHAPLTVDYDHKVKK