Protein Info for BPHYT_RS01440 in Burkholderia phytofirmans PsJN

Annotation: DNA topoisomerase III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 889 TIGR01056: DNA topoisomerase III" amino acids 3 to 629 (627 residues), 562.5 bits, see alignment E=7.7e-173 PF01751: Toprim" amino acids 5 to 131 (127 residues), 36.5 bits, see alignment E=6.7e-13 PF01131: Topoisom_bac" amino acids 153 to 595 (443 residues), 385.7 bits, see alignment E=4.4e-119 PF13342: Toprim_Crpt" amino acids 645 to 708 (64 residues), 60.3 bits, see alignment 2.2e-20 amino acids 756 to 816 (61 residues), 66.8 bits, see alignment 2e-22

Best Hits

KEGG orthology group: K03169, DNA topoisomerase III [EC: 5.99.1.2] (inferred from 90% identity to bgd:bgla_1g38380)

Predicted SEED Role

"DNA topoisomerase III, Burkholderia type (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T1K2 at UniProt or InterPro

Protein Sequence (889 amino acids)

>BPHYT_RS01440 DNA topoisomerase III (Burkholderia phytofirmans PsJN)
MSKALIIAEKPSVANDIARALGGFTKHDEYYESDEYVLSSAVGHLLEIAAPEDYEVKRGK
WSFANLPVIPPHFDLNPIAKSESRLKVLTKLLKRKDVDRLINACDAGREGELIFRLIAQH
AKAKQPVQRLWLQSMTAGSIRDGFARLRSDEEMQPLADAARCRSEADWLVGINGTRAMTA
FNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAEFVCSAGFYEGRWFDP
KFKRDEFDPEKRDSRLWALPAAETIVAACRGQIGTVTEESKPSTQLSPALFDLTSLQREA
NGRFGFSAKNTLGLAQALYEKHKVLTYPRTDARALPEDYMDTVKETLGMLKESNNYLPFA
KQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKNLSEPEQKLYDLVVKRFLAVFFPAA
EYKVTTRITEVVGHHFKTEGKVLVEPGWLQVYGREISGEDANLVPVQKDEKVKTDKIAAQ
QLVTKPPARYNEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLI
REGRDLIPTAKAFQLMTLLRGLGVKELTAPELTGEWEYKLSQMERGNLPRDAFMQEIARM
TQTIVKRAKEYDSDTIPGDYATLQTPCPNCGGQVKENYRRFACSKCEFSISKIPGGRQFE
IPEVEELLQNKTIGPLSGFRSKMGRPFSAILKLSLDDEIKNYKLEFDFGQDAGGEDGEPP
DFSDQQPVGACPKCKGRVFEHGMSYVCENSVANPKTCDFRSGKVILQQEIAREQMAKLLE
EGRTDLLTNFKSSRTGRNFKAFLVKQNDGKIGFEFEKKEPSAKTAAKTAAAKSAAKAAPE
AESDDGEVSVAVKAAPAAKKAAAKKAPATKTAAAKKAPAKKAAGRKTGS