Protein Info for BBR_RS16935 in Bifidobacterium breve UCC2003

Annotation: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01143: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
  • amino acids 32 to 478 (447 residues), 380.6 bits, E=4.9e-118
• PF01225: Mur_ligase
  • amino acids 32 to 96 (65 residues), 38.4 bits, E=1.9e-13
• PF08245: Mur_ligase_M
  • amino acids 115 to 304 (190 residues), 144.6 bits, E=5.8e-46
• PF02875: Mur_ligase_C
  • amino acids 326 to 414 (89 residues), 36.3 bits, E=8.5e-13

Best Hits

KEGG orthology group: K01929, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase [EC: 6.3.2.10] (inferred from 76% identity to bll:BLJ_1299)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.10

Protein Sequence (481 amino acids)

>BBR_RS16935 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase (Bifidobacterium breve UCC2003)
MVPMSVEEIAKAVDGRLVAGDGSAIEATETVTDSRQAGSGAVFVAIKGERVDGHDFVDKV
ADQGAAAAIVDHEVACTTMPQIVVEDTVAALGLLAKHNIDRRRQLPGAFDLIGLTGSVGK
TTTKDLLSALLSTLGPTVAPIGSFNNEIGLPLTALRVNADTRFFVAEMGANHIGEIARLT
RIAPPNTVIVLRVGVAHLGEFGSRERIAQAKSEIVQGLLPGGTAVLNADDDYVVPMAQLA
SGDVLWFGQGHTHDSEVHADHIAADAADHAEFTMTDAHGEQVAVHLGIPGRHNVMNALAA
ATVALRYGMPLTAIAQTLAAQHTISPHRMALSTVSRQGASFELIDDSFNANPDSMKAGLD
GLRAWNANDGRQPFRVALLGAMLELGADERELHASVGEYAAGLGLDAIITVGSPSDAHLN
VLAESLAEGATRGRAASVDCVQTIDEAERIVLDLAKNHTDTVVLLKGSHASGLSALAERW
A