Protein Info for BBR_RS13760 in Bifidobacterium breve UCC2003

Annotation: flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00612: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
  • amino acids 33 to 374 (342 residues), 409.6 bits, E=6e-127
• PF04551: GcpE
  • amino acids 37 to 376 (340 residues), 462 bits, E=5.7e-143

Best Hits

Swiss-Prot: 95% identical to ISPG_BIFLO: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K03526, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase [EC: 1.17.7.1] (inferred from 95% identity to blo:BL0098)

MetaCyc: 46% identical to (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin) (Escherichia coli K-12 substr. MG1655)
RXN-15878 [EC: 1.17.7.3]

Predicted SEED Role

"1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (EC 1.17.7.1)" in subsystem Isoprenoid Biosynthesis (EC 1.17.7.1)

MetaCyc Pathways

• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (11/12 steps found)
• taxadiene biosynthesis (engineered) (11/13 steps found)
• methylerythritol phosphate pathway I (8/9 steps found)
• methylerythritol phosphate pathway II (8/9 steps found)
• isoprene biosynthesis I (8/10 steps found)
• superpathway of ergosterol biosynthesis II (10/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.7.1 or 1.17.7.3

Protein Sequence (407 amino acids)

>BBR_RS13760 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (Bifidobacterium breve UCC2003)
MMNTLNTEKSAEKPFRKTGNPVELTSESPLHPRRKSHRIMVGSVPVGGGAPISVQSMTNT
LTANVPATLQQIAELTAAGCDIVRVAVPSQDDADALPEICRKSPVPIIADIHFQSKYVFQ
AIDAGCAAVRVNPGNIRKFNEVGPSICKAATDAGISLRIGVNAGSLDKELYAKYGGPTPE
ALVASALKEAHMFEDVGFHDFKISVKHHDVITMVETYRLLASKGDWPLHLGVTEAGPAWQ
GTIKSCLAFGALLAEGIGDTIRVSLSAPPVEEVKVGCKLLEYMGLRPRKFDIISCPSCGR
AQVDVIQLASAVTEGLKDVTAPIRVAVMGCIVNGPGEAREADLGVASGNGKGQIFIKGKV
IETVPEDQIVDTLLTKAQEIAAQMEADGLVPVNATGPVVVPIQHPNR