Protein Info for BBR_RS11535 in Bifidobacterium breve UCC2003

Annotation: replicative DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 486 PF00772: DnaB" amino acids 46 to 146 (101 residues), 115.8 bits, see alignment E=1.7e-37 TIGR00665: replicative DNA helicase" amino acids 46 to 476 (431 residues), 507.7 bits, see alignment E=1.3e-156 PF03796: DnaB_C" amino acids 221 to 475 (255 residues), 342.3 bits, see alignment E=3.3e-106 PF06745: ATPase" amino acids 222 to 382 (161 residues), 31.9 bits, see alignment E=1.7e-11 PF13481: AAA_25" amino acids 223 to 400 (178 residues), 56.6 bits, see alignment E=5.6e-19

Best Hits

Swiss-Prot: 56% identical to DNAB_LEIXX: Replicative DNA helicase (dnaB) from Leifsonia xyli subsp. xyli (strain CTCB07)

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 94% identity to blf:BLIF_0183)

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (486 amino acids)

>BBR_RS11535 replicative DNA helicase (Bifidobacterium breve UCC2003)
MAEGNDTSYQGGYSGGASYQGGADRGSRSGASGATAAGDPIFDRVPPHDDDAEMAVLGGM
LMSKDAIGEVSQTIDVTDFYQPKHQTIYNAIIDLFSASQPVDVVLVANQLLADGNLEKVG
GADYLHSLVASVPTAANAMYYAEIVHQRAILRNVIAAGTKIAQLGYSAEGSQAEDVVNLA
QSEIYEMSMGKVRQDYAAIGPVVHDALDQLDKLQNGLVQKGVPTGFRDIDDVTQGLQPGQ
MIVVAGRPAMGKSTLGIDFARAAALHHNMTSVVFSLEMSKVELAQRIISAETNIPMAALR
RADDITPERWNTLNNFWNKMQNAPFFIDDSPNMSLMEIRAKCRRLKQTNDLKLVVIDYLQ
LMTSGKAVESRQQEVSEFSRALKLLAKELEVPVVALSQLNRGPEMRNDKKPQLSDLRESG
SIEQDADVVFLVHRPDFYDKEDRPGEADIIMAKHRNGPTETFHLAFLGATSKFKDMPQDY
NANQGV