Protein Info for BBR_RS10725 in Bifidobacterium breve UCC2003

Annotation: Lead, cadmium, zinc and mercury transporting ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 821 transmembrane" amino acids 14 to 34 (21 residues), see Phobius details amino acids 54 to 71 (18 residues), see Phobius details amino acids 91 to 95 (5 residues), see Phobius details amino acids 319 to 338 (20 residues), see Phobius details amino acids 344 to 366 (23 residues), see Phobius details amino acids 770 to 793 (24 residues), see Phobius details amino acids 799 to 818 (20 residues), see Phobius details PF00122: E1-E2_ATPase" amino acids 215 to 373 (159 residues), 129.3 bits, see alignment E=1.8e-41 TIGR01494: HAD ATPase, P-type, family IC" amino acids 216 to 415 (200 residues), 98.5 bits, see alignment E=4.6e-32 PF00702: Hydrolase" amino acids 589 to 661 (73 residues), 34.6 bits, see alignment E=4e-12 PF08282: Hydrolase_3" amino acids 707 to 760 (54 residues), 21.7 bits, see alignment 2.4e-08

Best Hits

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (821 amino acids)

>BBR_RS10725 Lead, cadmium, zinc and mercury transporting ATPase (Bifidobacterium breve UCC2003)
MSGLIRFFTKILDICRLVPMLPVVVIAAIPLALLGDWRPWGNAIAMPVLGNPGVGQWLII
ALVAIIVIDTVRGMIDDLRHGQVGVDLLAVVAILSTVAVREYWASWAVMLMITSGEAIEE
YAQAKAEHSLTALMQAAPQTAHVVNLPGVGCRDAADEDDSSDGFRRVGSVPATSSPQSTS
PTAPLGEGNQVPPSRGADSRSETEGSWSAEAATHRFDTVPVEQVKLGDVLMVLPGETVPV
DGELLSGSATLDLSNINGEPVPREVFAGARVMSGAVNGSTALTMRATQVAANSQYQRILE
LVASAQESRPAVVKTADRLAVPFTVLSLAIAGIAWAVSGVPTRFAQVLVLATPCPLLIAA
PVAYIAGTGRLAAAGVLIKAQDVLENLGRVTQVFFDKTGTLTVKQPQVVRVEMLPGAKTR
LNEDHVLMMAGVVESYSVHILSKGIAKAGADAMARLRQRFADGQRLCPEPEASWPGHGRE
YPVVKNINEDSGKGVSGEVNGHAVRVGRLSFAAAGDDGFLTAEVAGVSRADGPRQEAADL
LSPLHGQSPLGGDTEAGSGRTIAGNTKEKETLRTRFGMLQPDEMASYVSVDGQLIARIVL
RDVPRANTKTALANLHELGVTKLAMLTGDKRASAEIIADEVGIDEVHAELFPEDKVAAVK
AATEASAAPAGRANHAASPVSDAVDGIRSDGSQPSRSRCSRAAAIRKPVTMMVGDGVNDA
PVLAVADIGVAMTDGTSTAASESAQVVIMNDDIAAVPRAIAIARRTKRVMLQAVIAGLVL
ATIGMIAAAFNLIPVVVGAFLQEAIDVVSILWALTALIDRD