Protein Info for BBR_RS10525 in Bifidobacterium breve UCC2003

Annotation: phosphoenolpyruvate carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00311: PEPcase
  • amino acids 34 to 459 (426 residues), 263.5 bits, E=4e-82
  • amino acids 462 to 917 (456 residues), 468.8 bits, E=3.6e-144
• PF14010: PEPcase_2
  • amino acids 494 to 621 (128 residues), 32 bits, E=5.2e-12

Best Hits

KEGG orthology group: K01595, phosphoenolpyruvate carboxylase [EC: 4.1.1.31] (inferred from 97% identity to blo:BL0604)

Predicted SEED Role

"Phosphoenolpyruvate carboxylase (EC 4.1.1.31)" in subsystem Fermentations: Mixed acid or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.31)

MetaCyc Pathways

• CO₂ fixation into oxaloacetate (anaplerotic) (2/2 steps found)
• mixed acid fermentation (11/16 steps found)
• nitrogen remobilization from senescing leaves (5/8 steps found)
• partial TCA cycle (obligate autotrophs) (5/8 steps found)
• C4 photosynthetic carbon assimilation cycle, NAD-ME type (6/11 steps found)
• ethene biosynthesis V (engineered) (16/25 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (2/7 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (6/14 steps found)
• formaldehyde assimilation I (serine pathway) (5/13 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (7/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (16/56 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.31

Protein Sequence (917 amino acids)

>BBR_RS10525 phosphoenolpyruvate carboxylase (Bifidobacterium breve UCC2003)
MATPEEQITPADAAIVTTGTGRKGPEEHDLPQSLKDDMDLCLKILRDVLGEYNPELLSTF
DTVRNYSVEASAEHFAELEDPNPAQDGLKEAVNVIDNMTLHDAQLLARAFATYFHLANLS
EENYRVSVLHERENNVSGEQAVDPINELTVAYHQLINEMGPAKAKELLDQLEFHPVFTAH
PTEARRKAVEGKIRRISELLGEYKILGGSDKKECLRRLYNEIDALFRTSPIALKKPTPVE
EADTILDIFDNTLFHTIPKVYRRFDDWILGDQAGLVEPVCPAFFHPGSWIGSDRDGNPNV
TAKVSRAVARKFSDHVIAALEEATRTVGRNLTMEAETTPPSAELKNLWSHQKEMSERLTD
KAALISTKEMHRAVMLVMADRLHYTIERDADLMYHSCDDFLNDLKVVQRSLAEAGAKRSA
YGPLQDLIWQTETFGFHMVEMEFRQHSVVHARALADIREHGLHGERGDLQPMTHEVLDTF
RALGAIQKRNGLKAARRYIISFTKSAQNIKDVYELNRLAFSHPEDVPTIDVIPLFEQLED
LQNSVDVLEEMIKIPEVQARLKATGNKLEVMLGYSDSSKDAGPTSATLALHSAQERIAKW
AESHDIDLTLFHGRGGAVGRGGGPANRAVLAQPVGSVKCRFKLTEQGEVIFARYGNPVLA
IRHVESVAAATLLQSAPSVEKRNTDMTKKYADMAAQLDEAAHNRFLDLLNTDGFAPWFSI
VTPLTEIGLLPIGSRPAKRGLGAKSLDDLRTIPWIFSWAQARINLAAWYGLGTACEKFGD
LETMRQAYEEWPLFSTFIDNIEMSIAKTDERIAKMYLALGDREDLNKKVLEEMELTRKWV
LDIVGDKWPLQHRHVLGQAIRIRSPYVDALSVTQVLALKSLRKKVDKEELSQSQQAGFIY
LILCTVSGVAAGLQNTG