Protein Info for Ac3H11_5000 in Acidovorax sp. GW101-3H11

Annotation: 2-isopropylmalate synthase (EC 2.3.3.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 566 TIGR00970: 2-isopropylmalate synthase" amino acids 5 to 546 (542 residues), 732.7 bits, see alignment E=1.4e-224 PF00682: HMGL-like" amino acids 31 to 309 (279 residues), 244.2 bits, see alignment E=1.8e-76 PF08502: LeuA_dimer" amino acids 420 to 546 (127 residues), 61.4 bits, see alignment E=8.7e-21

Best Hits

Swiss-Prot: 63% identical to LEU12_RALSO: 2-isopropylmalate synthase 2 (leuA2) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 85% identity to aaa:Acav_0030)

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165JK31 at UniProt or InterPro

Protein Sequence (566 amino acids)

>Ac3H11_5000 2-isopropylmalate synthase (EC 2.3.3.13) (Acidovorax sp. GW101-3H11)
MIAKPATKYQPIAPIALADRQWPSRSITRAPRWLSTDLRDGNQALFEPMNGERKMKLFAE
LVRIGFKEIEVGFPAASQTDFDFVRRLIDENLIPDDVTLMVMTQSREDLIARTVEAVQGA
PRAIVHLYNATAPAWRRIVFGMNVTQVMQLIAHHVGYLKQLTDTQPETQWTLQYSPETFS
ATELSVSLKACQTAIAAWNAGPGRPIIINLPTTVENATPNVFADQIEWMDRHLAPREHIV
LSVHPHNDRGTGVAAAELAMMAGADRVEGCLFGNGERCGNVDIVTLALNLYTQGVHPNLD
FSDITSVARIAEECTSLPVHPRHPYAGDLVFTAFSGSHQDAIKKGFAAQDPNALWEVPYL
PIDPADLGRTYDSVIRVNSQSGKGGIAFLLEREHGVVMPRRMQVEFSAVVQRQTDASEGE
MTGDALWSLFQATYLRAPAQAAITCHSHKLDEDGQGIELDVTIDGTRQTLRGQGNGPIAA
TVDALGLPLRVDHYEERATGTGANAQALAIVEAAMEGVAGATFGAGASHNIVTASVLAIV
GVANRLMARRPQATAGATAAPMAHVD