Protein Info for Ac3H11_2960 in Acidovorax sp. GW101-3H11

Annotation: Nicotinamide-nucleotide adenylyltransferase, NadM family (EC 2.7.7.1) / ADP-ribose pyrophosphatase (EC 3.6.1.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00125: cytidyltransferase-like domain
  • amino acids 5 to 59 (55 residues), 47 bits, 1.1e-16
• PF01467: CTP_transf_like
  • amino acids 7 to 65 (59 residues), 42.2 bits, E=9.6e-15
• PF00293: NUDIX
  • amino acids 206 to 338 (133 residues), 56.4 bits, E=3.3e-19

Best Hits

KEGG orthology group: K13522, bifunctional NMN adenylyltransferase/nudix hydrolase [EC: 2.7.7.1 3.6.1.-] (inferred from 78% identity to dia:Dtpsy_0679)

Predicted SEED Role

"Nicotinamide-nucleotide adenylyltransferase, NadM family (EC 2.7.7.1) / ADP-ribose pyrophosphatase (EC 3.6.1.13)" in subsystem NAD and NADP cofactor biosynthesis global or Nudix proteins (nucleoside triphosphate hydrolases) or NAD regulation (EC 2.7.7.1, EC 3.6.1.13)

MetaCyc Pathways

• NAD biosynthesis from nicotinamide (1/2 steps found)
• NAD salvage pathway IV (from nicotinamide riboside) (1/2 steps found)
• superpathway of NAD biosynthesis in eukaryotes (9/14 steps found)

KEGG Metabolic Maps

• Folate biosynthesis
• Lipopolysaccharide biosynthesis
• Nicotinate and nicotinamide metabolism
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-

Use Curated BLAST to search for 2.7.7.1 or 3.6.1.- or 3.6.1.13

Sequence Analysis Tools

See A0A165L3I1 at UniProt or InterPro

Protein Sequence (348 amino acids)

>Ac3H11_2960 Nicotinamide-nucleotide adenylyltransferase, NadM family (EC 2.7.7.1) / ADP-ribose pyrophosphatase (EC 3.6.1.13) (Acidovorax sp. GW101-3H11)
MYDTALLIGRFEPVHSGHLALLHEALRVAPQVIVVVGSAWQARSPKNPFTWQEREAMLRD
ALPPADRSRVRVLPVRDYYNEAVWVQAVRQGVAQHTQPGARMALVGHFKDATSSYLRAFP
SWALIDLPRQGSMDATTIRDAYFGATAATVDQALAPLAAQIPASTLAQLQAFAHTPHYAA
LQEEWRMLRGYRQAWSAAPYPPVFVTVDAVLRCQDHVLLIRRAHAPGKGQLAVPGGFIEQ
RETVWQSCLRELVEETHCHLPEATMRAALQSVAVFDHPDRSQRGRTITHAHYFDLGDAPF
PVVQADDDAMLVQWTRIDALAGMEEEFFEDHFHMLDHFLTITGAPLRG