Protein Info for Ac3H11_2957 in Acidovorax sp. GW101-3H11

Annotation: N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 431 TIGR01879: amidase, hydantoinase/carbamoylase family" amino acids 30 to 427 (398 residues), 431.9 bits, see alignment E=1.2e-133 PF04389: Peptidase_M28" amino acids 89 to 178 (90 residues), 24.1 bits, see alignment E=4.2e-09 PF01546: Peptidase_M20" amino acids 101 to 426 (326 residues), 105 bits, see alignment E=7.9e-34 PF07687: M20_dimer" amino acids 233 to 333 (101 residues), 31.3 bits, see alignment E=2.6e-11

Best Hits

Swiss-Prot: 52% identical to HYUC_RHIML: N-carbamoyl-L-amino-acid hydrolase (hyuC) from Rhizobium meliloti

KEGG orthology group: K06016, N-carbamoyl-L-amino-acid hydrolase [EC: 3.5.1.87] (inferred from 87% identity to pna:Pnap_4022)

MetaCyc: 52% identical to N-carbamoyl-L-amino-acid hydrolase subunit (Sinorhizobium meliloti CECT4114)
N-carbamoyl-L-amino-acid hydrolase. [EC: 3.5.1.87]

Predicted SEED Role

"N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87)" in subsystem Hydantoin metabolism (EC 3.5.1.87)

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.87

Use Curated BLAST to search for 3.5.1.87

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162DLU2 at UniProt or InterPro

Protein Sequence (431 amino acids)

>Ac3H11_2957 N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87) (Acidovorax sp. GW101-3H11)
VKELASQTPPENTMNTQVKPNIENLRINGQRLWDSLMELAQIGATPKGGVCRLTLTDLDK
QGRDLVTRWAREAGMTVTIDKIGNGFMRRPGRNNNLPPIMTGSHIDTQPTGGKFDGNYGV
LAGIEVVRTLNDHGIETEAPIEVAFWTNEEGSRFVPVMMGSGVFAKAFTLEHAYAATDTE
GKTVKGELERIGYIGDQEPGDHPIGAYFETHIEQGPVLEDNDKTIGVVSGVLGIRWFDCT
VTGMEAHAGPTPMALRKDAMLAATRIMQDVVAAAHRHPPHGRGTVGMVQVFPNSRNVIPG
RVKFSIDLRNSTDALVDAMAAEVKAFADQVAQEHGVQVHIEMVSSYPAQLFQPECVDAVG
RAAAKLGYSHMPAVSGAGHDAVYMAKLAPSGMIFIPCKDGISHNEIEDAKPEHIEAGCNV
LLHAMLERAGT