Protein Info for Ac3H11_2456 in Acidovorax sp. GW101-3H11

Annotation: ATP-dependent Clp protease ATP-binding subunit ClpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 782 TIGR02639: ATP-dependent Clp protease ATP-binding subunit ClpA" amino acids 3 to 740 (738 residues), 1103.5 bits, see alignment E=0 PF02861: Clp_N" amino acids 13 to 62 (50 residues), 54.6 bits, see alignment 5.4e-18 PF00004: AAA" amino acids 217 to 349 (133 residues), 47.1 bits, see alignment E=1.8e-15 amino acids 498 to 621 (124 residues), 46.2 bits, see alignment E=3.4e-15 PF17871: AAA_lid_9" amino acids 357 to 458 (102 residues), 90.4 bits, see alignment E=3.5e-29 PF07724: AAA_2" amino acids 492 to 653 (162 residues), 200.9 bits, see alignment E=8.2e-63 PF00158: Sigma54_activat" amino acids 496 to 618 (123 residues), 23.7 bits, see alignment E=2e-08 PF07728: AAA_5" amino acids 497 to 615 (119 residues), 49.4 bits, see alignment E=2.7e-16 PF10431: ClpB_D2-small" amino acids 659 to 739 (81 residues), 83.5 bits, see alignment E=5e-27

Best Hits

Swiss-Prot: 62% identical to CLPA_ECOLI: ATP-dependent Clp protease ATP-binding subunit ClpA (clpA) from Escherichia coli (strain K12)

KEGG orthology group: K03694, ATP-dependent Clp protease ATP-binding subunit ClpA (inferred from 97% identity to aaa:Acav_2010)

MetaCyc: 62% identical to ATP-dependent Clp protease ATP-binding subunit ClpA (Escherichia coli K-12 substr. MG1655)
Endopeptidase Clp. [EC: 3.4.21.92]

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpA" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.92

Use Curated BLAST to search for 3.4.21.92

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165KUP1 at UniProt or InterPro

Protein Sequence (782 amino acids)

>Ac3H11_2456 ATP-dependent Clp protease ATP-binding subunit ClpA (Acidovorax sp. GW101-3H11)
MIAQELEVSLHMAFVEARQQRHEFITVEHLLLALLDNPSAAEVLRACSANIDDLRSSLSN
FIKDNTPQVAGTDEVDTQPTLGFQRVIQRAIMHVQSTGNGKKEVTGANVLVAIFGEKDSH
AVYYLHQQGVTRLDVVNFIAHGIKKGEPPEPAKAENQAEGEEGGGGERSEKASPLEQFTQ
NLNQAAKDGKIDPLIGRHYEVERTIQILCRRRKNNPLLVGEAGVGKTAIAEGLAWRITQN
DVPEILAEAVVYSLDMGALLAGTKYRGDFEQRLKGVLKSLKDKPNAILFIDEIHTLIGAG
AASGGTLDASNLLKPALSSGQLKCIGATTFTEYRGIFEKDAALSRRFQKVDVVEPTVQET
IDILKGLKSRFEEHHSVKYAAAALQAAAELSAKYINDRHLPDKAIDVIDEAGAAQRILVP
SKRKKTIGKAEIEEIVAKIARIPPANVSNDDRSKLQTLERDLKSVVFGQDKALEVLASSV
KMARSGLGKGDKPIGSFLFSGPTGVGKTEAAKQLAYIMGIELIRFDMSEYMERHAVSRLI
GAPPGYVGFDQGGLLTEAITKKPHAVLLLDEIEKAHPDIFNVLLQVMDHGTLTDNNGRKA
DFRNVLIIMTTNAGAETMNKATIGFTNPRQAGDEMGDIKRLFTPEFRNRLDAIVNFKALD
EQIILRVVDKFLLQLETQLAEKKVEVTFTDTLRKHLAKKGFDPLMGARPMQRLIQDTIRR
ALADELLFGRLTEGGRLTVDIEMKTDDKGVETPEVLLDIQPLPKKERSAKSEPAEPEEAT
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