Protein Info for Ac3H11_2292 in Acidovorax sp. GW101-3H11

Annotation: Intracellular PHB depolymerase (EC 3.1.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 540 TIGR01849: polyhydroxyalkanoate depolymerase, intracellular" amino acids 2 to 418 (417 residues), 561.7 bits, see alignment E=4.2e-173 PF06850: PHB_depo_C" amino acids 213 to 418 (206 residues), 293.7 bits, see alignment E=3.1e-92

Best Hits

KEGG orthology group: K05973, poly(3-hydroxybutyrate) depolymerase [EC: 3.1.1.75] (inferred from 76% identity to vei:Veis_0272)

Predicted SEED Role

"Intracellular PHB depolymerase (EC 3.1.1.-)" in subsystem Polyhydroxybutyrate metabolism (EC 3.1.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.-

Use Curated BLAST to search for 3.1.1.- or 3.1.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162F7G5 at UniProt or InterPro

Protein Sequence (540 amino acids)

>Ac3H11_2292 Intracellular PHB depolymerase (EC 3.1.1.-) (Acidovorax sp. GW101-3H11)
MLYHIYETQRSLMEPFTDFAQAASKLFSNPVSPLSQTSAAQRMAAGYDLLYRLGKDYEKP
AFDIHTVDVNGIGVAIHERIEVNKPFCELRRFKRFSDDPATLTKLKGQPVVLIVAPLSGH
YATLLRDTVRTMLKDHKVYITDWKNARLVPLAEGEFHLDDYVNYVQEFIRHLQGQYGNCH
VISVCQPTVPVLAAVSLMASRGEKTPLTMTMMGGPIDARKSPTSVNNLATNRSYEWFENN
VIYRVPDKFPGAGRRVYPGFLQYTGFVAMNPDRHANSHYDYFKDLIKGDDASAEAHRKFY
DEYNAVLDMDADYYLETIQTVFQDYKLVNGTWDVRSPTGKIERVRPQDITTTALFTVEGE
LDDISGSGQTEAAHDLCSGIVRKEQRHLEAKGAGHYGIFSGRRWRDLVYPKVRAFILEHE
LPRAPAAPLAAAPVAKAATPAVAVAAPSAKPKAAPQPKPAAKKAQAAKAAPSAPRARTAA
AAKTTAPAATVATRWVKTEGAAQPTGAAEQAPADLGAAKATAVTAAPARSASRSSKARKA