Protein Info for Ac3H11_2027 in Acidovorax sp. GW101-3H11

Annotation: Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 638 transmembrane" amino acids 477 to 498 (22 residues), see Phobius details PF13667: ThiC-associated" amino acids 26 to 95 (70 residues), 78.4 bits, see alignment E=3e-26 TIGR00190: phosphomethylpyrimidine synthase" amino acids 144 to 600 (457 residues), 714.7 bits, see alignment E=1.7e-219 PF01964: ThiC_Rad_SAM" amino acids 145 to 597 (453 residues), 625.9 bits, see alignment E=3.1e-192

Best Hits

Swiss-Prot: 91% identical to THIC_ACIAC: Phosphomethylpyrimidine synthase (thiC) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 86% identity to vei:Veis_1523)

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165KP37 at UniProt or InterPro

Protein Sequence (638 amino acids)

>Ac3H11_2027 Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17) (Acidovorax sp. GW101-3H11)
MNAPDPHAQLAAPEKFAELLARTREPFPASRKAYIPGQLHTDLRVPVRDIALTNGELVSV
YDTSGPYTDPNAVIDVRQGLASVRGGWITGRGDVEHYEGRAPVALDDGQKSEDATRLAQL
RAEAAALQRKPLRAKSGANHTGNVTQMHYAKKGIITPEMEYVAIRENGKREWMEQYMADA
GREQRLMGNPMGASIPRIITPEFVRDEVARGRAIIPANINHPEVEPMAIGRNFLVKINAN
IGNSAVTSSIEEEVEKLVWAIRWGADNVMDLSTGKNIHTTRDWIVRNSPVPIGTVPIYQA
LEKVGGVAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLAYIHLTAQRRTGIVSRGGSIM
AKWCMAHHRESFLYEHFEDICDIMKAYDVSFSLGDGLRPGCASDANDEAQFAELHTLGEL
TQVAWKHDVQTMIEGPGHVPMHMIQANMTEQLKTCHEAPFYTLGPLTIDIAPGYDHIASA
IGAAMIGWMGTAMLCYVTPKEHLGLPDRDDVKQGIIAYKIAAHAADVAKGHPGARSRDDA
LSQARFDFRWQDQFNLGLDPETAKEYHDETLPKDSAKVAHFCSMCGPKFCSMKITQEVRD
FAKQKGVAEADALAQGMETKAAEFQRGGGELYIPLKPV