Protein Info for Ac3H11_1817 in Acidovorax sp. GW101-3H11

Annotation: tRNA(Cytosine32)-2-thiocytidine synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 313 PF01171: ATP_bind_3" amino acids 54 to 211 (158 residues), 48.1 bits, see alignment E=5.8e-17

Best Hits

Swiss-Prot: 86% identical to TTCA_ACIAC: tRNA-cytidine(32) 2-sulfurtransferase (ttcA) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K14058, tRNA 2-thiocytidine biosynthesis protein TtcA (inferred from 86% identity to aav:Aave_4231)

MetaCyc: 68% identical to tRNA cytosine32 2-sulfurtransferase TtcA (Escherichia coli K-12 substr. MG1655)
2.8.1.M2 [EC: 2.8.1.M2]

Predicted SEED Role

"tRNA(Cytosine32)-2-thiocytidine synthetase"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.M2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162DLV1 at UniProt or InterPro

Protein Sequence (313 amino acids)

>Ac3H11_1817 tRNA(Cytosine32)-2-thiocytidine synthetase (Acidovorax sp. GW101-3H11)
MSTVWTEVEAPSVAATAPRNDLKIERETHKLEKRLCREVGKAIVDFNMIEEGDKVMVCMS
GGKDSYTLLDILIKMKARAPIHFDLVAVNLDQKQPGFPEHILPEYLASTGVPFHIENEDT
YSIVKKLVPEGKTTCSLCSRLRRGILYRVADELGCTKIALGHHRDDILQTLLLNMFFGGK
MKSMPPKLVSDDGKHVVIRPLAYVAEKDTARWSAHRDFPIIPCNLCGSQENLQRKQVGEM
LREWEKKFPGRVENMFTALQNVVPSHLLDGDLYDFKNARATGIASEDGDKAFDKEEFAAP
SPSLPGVQVVQLS