Protein Info for Ac3H11_1528 in Acidovorax sp. GW101-3H11

Annotation: 3-isopropylmalate dehydrogenase (EC 1.1.1.85)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 90% identical to LEU3_RHOFT: 3-isopropylmalate dehydrogenase (leuB) from Rhodoferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118)

KEGG orthology group: K00052, 3-isopropylmalate dehydrogenase [EC: 1.1.1.85] (inferred from 94% identity to aav:Aave_1221)

MetaCyc: 60% identical to beta-isopropylmalate dehydrogenase subunit (Leptospira interrogans serovar Lai str. 56601)
3-isopropylmalate dehydrogenase. [EC: 1.1.1.85]; 1.1.1.- [EC: 1.1.1.85]

Predicted SEED Role

"3-isopropylmalate dehydrogenase (EC 1.1.1.85)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 1.1.1.85)

MetaCyc Pathways

superpathway of branched chain amino acid biosynthesis (17/17 steps found)
L-leucine biosynthesis (6/6 steps found)
3-methylbutanol biosynthesis (engineered) (6/7 steps found)
L-isoleucine biosynthesis II (5/8 steps found)
butanol and isobutanol biosynthesis (engineered) (5/8 steps found)
aliphatic glucosinolate biosynthesis, side chain elongation cycle (10/30 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.85

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165K8E4 at UniProt or InterPro

Protein Sequence (357 amino acids)

>Ac3H11_1528 3-isopropylmalate dehydrogenase (EC 1.1.1.85) (Acidovorax sp. GW101-3H11)
MKIAVLPGDGIGTEIVAEAVKVLKALDLKFEMESALVGGAAYEAHGHPLPESTLKLAKEA
DAILFGAVGDWKYDKLDRPLRPEQAILGLRKNLGLFANFRPAICYEQLVGASSLKPELIA
GLDILIIRELTGDIYFGQPRGRRVATDGHFPGAEEAFDTMRYSRPEIERIAHVAFQAARK
RNKKVTSVDKANVLETFQFWKDVVTDVHKEYPDVELQHMYVDNAAMQLVKAPKAFDVVVT
GNMFGDILSDEASMLTGSIGMLPSASLNSSNQGLYEPSHGSAPDIAGKGVANPLATILSA
AMMLRFSLNQEAAAQRIEAAVQKVLAQGLRTPDIYSEGTTKVGTAQMGDAVVKALAA