Protein Info for AZOBR_RS32325 in Azospirillum brasilense Sp245

Updated annotation (from data): Fructose PTS system (E-I, HPr, and E-IIA components)
Rationale: Specifically important for utilizing D-Fructose. This gene includes components E-I (PEP-protein phoshpotransferase), HPr, and E-IIA. Components E-IIB and E-IIC are in the adjacent gene AZOBR_RS32335.
Original annotation: PTS fructose transporter subunit IIA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 846 PF00359: PTS_EIIA_2" amino acids 14 to 150 (137 residues), 99.1 bits, see alignment E=5.7e-32 PF00381: PTS-HPr" amino acids 170 to 248 (79 residues), 70 bits, see alignment E=3.6e-23 TIGR01003: phosphocarrier, HPr family" amino acids 173 to 247 (75 residues), 51.3 bits, see alignment E=8.8e-18 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 283 to 841 (559 residues), 459 bits, see alignment E=2.2e-141 PF05524: PEP-utilisers_N" amino acids 283 to 401 (119 residues), 77.8 bits, see alignment E=2e-25 PF00391: PEP-utilizers" amino acids 431 to 502 (72 residues), 73 bits, see alignment E=3.3e-24 PF02896: PEP-utilizers_C" amino acids 528 to 817 (290 residues), 363.6 bits, see alignment E=1.8e-112

Best Hits

Swiss-Prot: 69% identical to PTFAX_XANCP: Multiphosphoryl transfer protein (fruB) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 78% identity to azl:AZL_c02630)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8B0J1 at UniProt or InterPro

Protein Sequence (846 amino acids)

>AZOBR_RS32325 Fructose PTS system (E-I, HPr, and E-IIA components) (Azospirillum brasilense Sp245)
MAPDANTAIMPTDLLRPELLRLSASPAGKEEAIREAAQLLIAAGCIDSAYAASMLRREAV
ANTFLGHGVAIPHGMVEDRGMVRRSGIAVLQVPGGIAWNPGQTAHLVVAIAAQSDAHIAV
LRRLTRLMQDEARLTALFTVTDPAALAAALGEDAPVAAPATPGGDLAERFDWVVDYPTGL
HARPATAWVETARASAARIQVRHGDLVADAKALVALLQLGLRAGDSVVVSAEGDDAVAAL
ARMKATITRLTAREKADAAAAAQKARAPVRGWTPPNPLPAVPGIAASPGLAIGPVHVLPR
AAVSVPDEPVPLIEGGDRLHEALSLTRQNLKALADDTARRLGPSEAAIFAAQAEILNDTD
LVTLACQLMVEGHGVAWSWHQAVERTAAGLAALDNPVLAARAADLRDVGQRVLARIDPAL
RTGGAPDLPDTPCILIAEDLSPSDTAALDMARVIGLATAQGGPTSHTAILARTLGLPAMV
AGGAALMELANGTPAILDGQSGRLHLSPAAADIADARAWIAREEARKAEEEARRGLPART
RDGHEVEIGANVNRPDQVAVALSQGAESVGLMRTEFLFLERGDAPGEDEQYETYRGMLTA
LEGRPLIVRALDIGGDKQVPHLQLPHEENPFLGVRGARLLLRRPELLETQLRALYRAAKD
GGAKDGGALSIMFPMITALGEVQALRAACERIRAELDAPAVPLGIMVEVPAAAIQADVLA
RHVDFFSIGTNDLTQYALAIDRQHPELAAEADSLHPAVLRLIRLTVEGAERHGRWVGVCG
GIAGDPFGAALLTGLGVRELSMTPRDIPAVKDRLRGSDLSALKDAAQRALDCETADAVRA
LDGGAA