Protein Info for AZOBR_RS28125 in Azospirillum brasilense Sp245
Annotation: 3-carboxy-cis cis-muconate cycloisomerase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 58% identical to TAD1_USTMD: Trans-aconitate decarboxylase 1 (TAD1) from Ustilago maydis
KEGG orthology group: K01857, 3-carboxy-cis,cis-muconate cycloisomerase [EC: 5.5.1.2] (inferred from 80% identity to lch:Lcho_2418)MetaCyc: 58% identical to trans-aconitate decarboxylase (Ustilago maydis)
RXN-19902 [EC: 4.1.1.113]
Predicted SEED Role
"3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 5.5.1.2)
MetaCyc Pathways
- protocatechuate degradation II (ortho-cleavage pathway) (3/4 steps found)
- itaconate biosynthesis II (1/3 steps found)
- aromatic compounds degradation via β-ketoadipate (4/9 steps found)
- toluene degradation III (aerobic) (via p-cresol) (3/11 steps found)
- superpathway of aerobic toluene degradation (6/30 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (8/35 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.1.1.113 or 5.5.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See G8AXX3 at UniProt or InterPro
Protein Sequence (453 amino acids)
>AZOBR_RS28125 3-carboxy-cis cis-muconate cycloisomerase (Azospirillum brasilense Sp245) MPSTIIDSAIFGDIFSTPAMRAVWSDENRTRKYLDIEAALARVQGRLGIIPQEAADEIVA NCSLDRIDMATLKAQTERIGYPVLGVVSQLNALCRDRLGEYCHWGATTQDITDTATVLQM REALNLVDEDLAGLSAALATLAKRHRDTPMIGRSNLQQAVPVTFGYKMAGLLSAVERHRE RLAQLRPRVLMGEFAGASGTLASLEHGAMETQAGLMEELDLAQPVIAWHTIRDTIAEVGC FLGLVGGTLGKLSMDVKLMMQTEVGEVYEPFAHGRGSSSTMPQKRNPISSCYIHASISVL RQHTAALLDAMVADHERSTGPWEIEWIVLPEAFCLLAGALKQARFVVSGLEVDAERMRAN LEMTNGLVASEAVMMGLGRHIGREYAHDLVYDLCREAVRQNRPLLDLLAETPEISACLSR AELAKLCDPANYLGQSGVMVDRVLDRVKNPVRG