Protein Info for AZOBR_RS26025 in Azospirillum brasilense Sp245

Annotation: alpha gluctosidase galactosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 431 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF02056: Glyco_hydro_4" amino acids 10 to 189 (180 residues), 144.1 bits, see alignment E=3.6e-46 PF11975: Glyco_hydro_4C" amino acids 201 to 406 (206 residues), 124.9 bits, see alignment E=4.7e-40

Best Hits

KEGG orthology group: K07406, alpha-galactosidase [EC: 3.2.1.22] (inferred from 79% identity to azl:AZL_e01400)

Predicted SEED Role

"Alpha-galactosidase (EC 3.2.1.22)" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Melibiose Utilization (EC 3.2.1.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AWC8 at UniProt or InterPro

Protein Sequence (431 amino acids)

>AZOBR_RS26025 alpha gluctosidase galactosidase (Azospirillum brasilense Sp245)
MAVTTRKTTKIVFLGAGSAAFGLSMYRDLFTTTELAGSTLTLVDTNPAALDRMTALARLL
NAKGDAGLIIEQTTDRRAALQGAEFVVNSVAIDRNRLWKLDFEVPKKHGIRHTLGENGGP
GGLFFTLRTLPLIFDFARDIEELCPEAQFINLSNPESRVVLGLGKYTKVKTLGLCHGIFL
ARWFICQILGMAEKDVEVWGAGLNHFQWLMHIRDKESGRDLYPLLREREKTHDPAFTPLT
RRLFNSFGLWPTCSDDHMGEYLPYGWEGGEHGFDFAEDERGRGILNALMDGTIAGTTPVP
DDWTQPSWGERAVQVIAGVLHNQKRFIESGIVYNQGAIPNLPPDLAVEVPLLADAAGVHP
VSLGPLPDPVAKLLHVQASVQQLSVEAAVHASKDLALQALLIDPVVNSTDAAVKILDELW
EANRPYIRPCV