Protein Info for AZOBR_RS20745 in Azospirillum brasilense Sp245

Annotation: ubiquinol-cytochrome C reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 189 transmembrane" amino acids 26 to 47 (22 residues), see Phobius details PF10399: UCR_Fe-S_N" amino acids 17 to 53 (37 residues), 70.1 bits, see alignment 8.3e-24 TIGR01416: ubiquinol-cytochrome c reductase, iron-sulfur subunit" amino acids 22 to 189 (168 residues), 234.2 bits, see alignment E=1e-73 TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 22 to 46 (25 residues), 21.8 bits, see alignment (E = 1.8e-08) PF00355: Rieske" amino acids 114 to 175 (62 residues), 33.7 bits, see alignment E=2.8e-12

Best Hits

Swiss-Prot: 71% identical to UCRI_RHORU: Ubiquinol-cytochrome c reductase iron-sulfur subunit (petA) from Rhodospirillum rubrum

KEGG orthology group: K00411, ubiquinol-cytochrome c reductase iron-sulfur subunit [EC: 1.10.2.2] (inferred from 88% identity to azl:AZL_c04850)

Predicted SEED Role

"Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2)" in subsystem Ubiquinone Menaquinone-cytochrome c reductase complexes (EC 1.10.2.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.10.2.2

Use Curated BLAST to search for 1.10.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AP22 at UniProt or InterPro

Protein Sequence (189 amino acids)

>AZOBR_RS20745 ubiquinol-cytochrome C reductase (Azospirillum brasilense Sp245)
MAQTTHPPGTGGHAAPGGEGASRRDFLYLATGAVGAIGVASAVWPFIDSMNPAADTLALA
SIDVDLAPVQEGQAITVTWRGKPVFVRHRTAKEIEEARTVNLGELRDPAADDARVKKPEW
LIVVGICTHLGCVPLGQKPTDPRGDYDGWFCPCHGSHYDTAGRIRKGPAPANLVVPQYAF
TSDTAVRLG