Protein Info for AZOBR_RS20050 in Azospirillum brasilense Sp245

Annotation: putative protease-like protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 662 PF08548: Peptidase_M10_C" amino acids 266 to 345 (80 residues), 25.6 bits, see alignment E=1.1e-09 PF00353: HemolysinCabind" amino acids 333 to 343 (11 residues), 9.5 bits, see alignment (E = 0.00011) amino acids 360 to 383 (24 residues), 24.7 bits, see alignment (E = 1.9e-09) amino acids 377 to 400 (24 residues), 24.2 bits, see alignment (E = 2.6e-09) amino acids 517 to 551 (35 residues), 15.9 bits, see alignment (E = 1e-06) amino acids 525 to 560 (36 residues), 21 bits, see alignment 2.7e-08 amino acids 544 to 569 (26 residues), 17.9 bits, see alignment (E = 2.6e-07)

Best Hits

Predicted SEED Role

"Alkaline phosphatase (EC 3.1.3.1)" in subsystem Phosphate metabolism (EC 3.1.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.1

Use Curated BLAST to search for 3.1.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8ANL9 at UniProt or InterPro

Protein Sequence (662 amino acids)

>AZOBR_RS20050 putative protease-like protein (Azospirillum brasilense Sp245)
MSIAAWGPTSSATVYGLTAANRPTTDIDGGPLGCDPALLRTGIVTTPQTISDTSLIPILS
GSKWGTAQAAAPVTLTYSFYESGGPAVAAAGVTPAPLDIAFRDAIRSALVSIADVANISF
VEITESATGGATGAPGASGALVGHLRFIDDARLDSDPNTASYAIGPSATALSSGLVAFDS
DRVTAASGGGYATILREIGRALGLTYSFEPSDAPATYRGPTSENGVWSTEWTMMSATMPV
SRLPAGAVFNPAGPMMADILVLQSWYGANLNTRTGNDTYRIDGPLLTRALWDAGGIDTLD
ASASTTGVNFNLEASSINYGHNLAPRALFENAIGSAGNDTIFGTNERPSIGPDGVWFDHS
GNNRIDGGPGDDQILAGSGNDTLIGGTGRNSLSGGLGNDTAVYDAASTDAYAYTVQGTLI
LSIPKLGISDTLVDIEQLSFTDRSFAAADATSILLPSSANPNPPSSPIPPSLTPPPSPVP
AIDPARRLYVTRGGASTEVAMDSYSGPVTWLRNMHIGQNDSEAMHGTAHADFINTLGGDD
AVNGGGGDDVLDGGLGSNYLIGGSGSDTFFVDGRGGGITWSTVTDLEPGEWVTAWGWKEG
TSKLTWAEMAGAEGAKGATAHIDLDNNGSIDMSMTIAGKSSGAVLVTPGEVNGSSYLAFT
LK