Protein Info for AZOBR_RS16895 in Azospirillum brasilense Sp245

Annotation: beta-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 482 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 3 to 27 (25 residues), 17.4 bits, see alignment (E = 4.3e-07) PF00232: Glyco_hydro_1" amino acids 39 to 477 (439 residues), 479.7 bits, see alignment E=3.7e-148 TIGR03356: beta-galactosidase" amino acids 40 to 469 (430 residues), 573.9 bits, see alignment E=2.1e-176

Best Hits

KEGG orthology group: K05350, beta-glucosidase [EC: 3.2.1.21] (inferred from 71% identity to azl:AZL_e01630)

Predicted SEED Role

"Beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism or Fructooligosaccharides(FOS) and Raffinose Utilization (EC 3.2.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8ARQ1 at UniProt or InterPro

Protein Sequence (482 amino acids)

>AZOBR_RS16895 beta-glucosidase (Azospirillum brasilense Sp245)
MRRRSFLKAAAATGLAASAGAFLTTRRAAVAQPSEVLAAFPDKFVWGASTSSYQIEGAVT
AGGRGPSVWDTFSHSFGKVANGDTGDVACDHYNRYAEDVDLMAKAGMNAYRFSVAWPRVQ
PTGTGPANAEGLDFYDRLTDALLAKGIAPWPCLYHWDLPQALQDRGGWTNRDIAGWFTDY
AQLVAARIGDRAKHWTMLNEPSVHAIFGHGLGGHAPGMTGKGNYFKAIHHQNLAQGMALK
ALRAAGGAKGWQLGTVLSLQPVWPVGGLDANYAASLMWDAVWNRACLDPLLRGEYPELLR
DGFAPLVKAGDLEAIRQPIDFLGINYYSRMHQQPDPAGLFGTGYGSPPEGTPTTGMGWPV
EPDGIAEILIELKQEYGNPPVYVMENGAAYPEQTGPKGFVQDNDRISYLRRHILAGHQAL
EEGVDLRGWFVWSLLDNFEWAEGYQRRFGLIEVDRQTLERRPKASYHWYADVIRKKGVPT
SA