Protein Info for AZOBR_RS16640 in Azospirillum brasilense Sp245

Annotation: 3-hydroxybutyrate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 261 TIGR01963: 3-hydroxybutyrate dehydrogenase" amino acids 5 to 261 (257 residues), 352.8 bits, see alignment E=4.7e-110 PF00106: adh_short" amino acids 6 to 197 (192 residues), 188 bits, see alignment E=2e-59 PF08659: KR" amino acids 9 to 161 (153 residues), 40.9 bits, see alignment E=3.2e-14 PF13561: adh_short_C2" amino acids 14 to 259 (246 residues), 193.3 bits, see alignment E=7.9e-61

Best Hits

Swiss-Prot: 66% identical to BDHA_CUPNH: D-beta-hydroxybutyrate dehydrogenase (hbdH1) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00019, 3-hydroxybutyrate dehydrogenase [EC: 1.1.1.30] (inferred from 73% identity to rce:RC1_1846)

MetaCyc: 42% identical to aclacinomycin polyketide synthase reductase (Streptomyces galilaeus)
1.1.1.-

Predicted SEED Role

"D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30)" in subsystem Polyhydroxybutyrate metabolism (EC 1.1.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.30

Use Curated BLAST to search for 1.1.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8ARJ6 at UniProt or InterPro

Protein Sequence (261 amino acids)

>AZOBR_RS16640 3-hydroxybutyrate dehydrogenase (Azospirillum brasilense Sp245)
MTLTQKVAVVTGSTSGIGLGIARALAGAGADVVLNGFGEAAAIEELRAGLAAEFGVRVGY
HGADLSKPAEIAALIGHAEETFGSVDVLVNNAGIQHVAPVEDFPAERWDAVIALNLSAVF
HGTHHALPGMKRRGWGRILNIASVHGHVASVNKSAYVAAKHGVVGLTKTVALETAGTGVT
CNAICPGWVLTPLVQKQIDAIASTKNIPEPQAKAELLGAKQPSGAFVTPDELGGLAVFLC
SDSAAQMTGASLLMDGGWTAQ