Protein Info for AZOBR_RS16015 in Azospirillum brasilense Sp245

Annotation: 3-phosphoglycerate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 336 PF00389: 2-Hacid_dh" amino acids 26 to 334 (309 residues), 81.2 bits, see alignment E=8.8e-27 PF02826: 2-Hacid_dh_C" amino acids 116 to 303 (188 residues), 155.4 bits, see alignment E=1.5e-49 PF03446: NAD_binding_2" amino acids 154 to 242 (89 residues), 22.6 bits, see alignment E=1.4e-08

Best Hits

KEGG orthology group: K00058, D-3-phosphoglycerate dehydrogenase [EC: 1.1.1.95] (inferred from 87% identity to azl:AZL_b00610)

Predicted SEED Role

"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.95

Use Curated BLAST to search for 1.1.1.95

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AR49 at UniProt or InterPro

Protein Sequence (336 amino acids)

>AZOBR_RS16015 3-phosphoglycerate dehydrogenase (Azospirillum brasilense Sp245)
MTMGKRAPFVVGITDHMIPPPDLEAAVLDGCAEVDFLDTRREEDLDPERLARLDALLVWS
TRIGPATVAKLPRCRVVVRFGVGYDKVDVAALEAAGIPFCNNPDYGTEEVADHAVSLLLS
LQRRLWEHDARARGYSTTWQANTLTPLRRSSAATVGVVGVGRIGTAVVNRLKPFGYRILG
YDPQQPAGHEKAVGYRRVRRLDELLAESDIVTFHCPLTPETRGLIDADFLAKLKPGALLV
NTARGEMFAGLDPLEAALRSGHVAAVGTDVLPTEPPAPHPLLDAWRRRESWLDGRLVVTP
HNAFHSDEAAIEMRRNAAETARLFLEDRVLRNRILP