Protein Info for AZOBR_RS14515 in Azospirillum brasilense Sp245

Annotation: oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 485 transmembrane" amino acids 6 to 23 (18 residues), see Phobius details amino acids 30 to 48 (19 residues), see Phobius details amino acids 61 to 86 (26 residues), see Phobius details amino acids 98 to 117 (20 residues), see Phobius details amino acids 123 to 142 (20 residues), see Phobius details amino acids 154 to 176 (23 residues), see Phobius details amino acids 196 to 217 (22 residues), see Phobius details amino acids 237 to 256 (20 residues), see Phobius details amino acids 268 to 290 (23 residues), see Phobius details amino acids 310 to 331 (22 residues), see Phobius details amino acids 370 to 395 (26 residues), see Phobius details amino acids 405 to 425 (21 residues), see Phobius details amino acids 455 to 480 (26 residues), see Phobius details PF00361: Proton_antipo_M" amino acids 118 to 412 (295 residues), 167.4 bits, see alignment E=2.2e-53

Best Hits

Swiss-Prot: 59% identical to HYFF_METI4: Hydrogenase-4 component F homolog (hyfF) from Methylacidiphilum infernorum (isolate V4)

KEGG orthology group: K12141, hydrogenase-4 component F [EC: 1.-.-.-] (inferred from 73% identity to rce:RC1_1417)

Predicted SEED Role

"Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AQ43 at UniProt or InterPro

Protein Sequence (485 amino acids)

>AZOBR_RS14515 oxidoreductase (Azospirillum brasilense Sp245)
LSAVAVIVATPLIGAAVLALVPGHRLGARLNIGFSAVTLLASLVLFGLEPSSGALFVVDA
FNIYLIALTAFVGLTTSVFSAGYIAHEIAVGKLDERKLRFYHAMYQAFMFTMLLALSANN
LGVMWVAVEGATLTTVLMVSLYRTAASIEAAWKYFILCGVGIALALFGTILMYMAAQPVM
GPGMAAMTWTELMGHVAKISPAILNLAFVFLLIGYGTKVGLAPLHAWLPDAHAEGPTPIS
AVLSGLLLNVALYAVLRFKMLLAANGQAIAPGPLMIAMGLASLLLAGLMLYRRRDVKRFF
AYSSIEHMGIITFAFGMGGPLANFAGLLHMAMHSLTKSAIFYAVGHAVQVTGTQRIERIS
GLTVSHPALGWGLLAGVVAIAGLPPFGVFMSEFLLVTSTFAREPLLALPLVVGILVAFGA
LVLRVQQLCFGAPGTVEGSGVPVASVPLEGTLVPLYAHLALVLVAGVWLPGPLVAWFQTV
AALLG