Protein Info for AZOBR_RS14045 in Azospirillum brasilense Sp245

Annotation: lysyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 522 PF01921: tRNA-synt_1f" amino acids 12 to 365 (354 residues), 452.2 bits, see alignment E=5.7e-140

Best Hits

Swiss-Prot: 70% identical to SYK_CAUSK: Lysine--tRNA ligase (lysS) from Caulobacter sp. (strain K31)

KEGG orthology group: K04566, lysyl-tRNA synthetase, class I [EC: 6.1.1.6] (inferred from 89% identity to azl:AZL_025690)

Predicted SEED Role

"Lysyl-tRNA synthetase (class I) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.6

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AHS8 at UniProt or InterPro

Protein Sequence (522 amino acids)

>AZOBR_RS14045 lysyl-tRNA synthetase (Azospirillum brasilense Sp245)
MTGERELALQAKAWPFEEARKLVARFAKEPPAKGYVLFETGYGPSGLPHIGTFGEVARTT
MVRQAFQRMSDIPTKLFCFSDDMDGLRKVPDNIPNKELVAASLGKPLTQVPDPFGTHDSF
GAHNNARLRAFLDSFGFEYEFQSSTDWYKSGRFDEALLGVLRRYDEIMAVMLPTLGEERQ
QTYSPFLPVSPSTGRVLQVPVLERNVDAGTIVFQDEDGKKVEVPVTGGHVKLQWKPDWGM
RWYALGVDYEMYGKDLIPSAELAGKIVNILGGTPPQGFNYELFLDDKGQKISKSKGNGLT
MEEWLAYAPPESLALYMFQKPKSAKRLYFDVIPRAVDEYLAFVAKLPVEEPAKALENPAW
HIHNGTAPAVRSDVSFNLLLNLAGAANAETKDVMWGFISRYAPEATPENSPFLDKLVGYA
VRYYQDQVKPTKRFRAPTDAERAALEDLLKRLDGIPADAAGDVIQNEVFAVGKEHGFTEL
RAWFQALYEVLLGQTTGPRMGSFIQLYGLDETKTLIREKLAA