Protein Info for AZOBR_RS11340 in Azospirillum brasilense Sp245

Annotation: O-acetylhomoserine aminocarboxypropyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 8 to 430 (423 residues), 591.3 bits, E=4.6e-182
• PF01053: Cys_Met_Meta_PP
  • amino acids 10 to 430 (421 residues), 423.8 bits, E=2.4e-131

Best Hits

Swiss-Prot: 49% identical to FUB7_FUSO4: Sulfhydrylase FUB7 (FUB7) from Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936)

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 87% identity to azl:AZL_006110)

MetaCyc: 49% identical to O-acetylhomoserine aminocarboxypropyltransferase (Rhodospirillum rubrum)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• L-methionine biosynthesis I (4/5 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (3/5 steps found)
• homocysteine and cysteine interconversion (2/4 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (2/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Sequence Analysis Tools

See G8ANJ9 at UniProt or InterPro

Protein Sequence (436 amino acids)

>AZOBR_RS11340 O-acetylhomoserine aminocarboxypropyltransferase (Azospirillum brasilense Sp245)
MADAKFLKFDTLSLHAGQRPDPATGARAVPIYQSTSYVFDDTDHAASLFNLERPGHIYSR
ISNPTVAVLEERLAALDGGVGAVCTASGQAALTLAIMTLMDAGGHIVASSSIYGGSRNLL
AYTLPRFGITTTFVNPRDLDGFRAAIRPETRLLFGEVLGNPGLEVLDIPSLSAIAHEAGL
PLMVDATFVTPYLCKPLSVGADLVMHSCTKWLAGHGVAIGGVVIDGGTFDWEASGKFPTL
TEPYAGYHGIDFAEEYGPAAFVMRARAEGLRDFGACMSPMNAFQILQGVETLPLRMKGHI
HNTRKVLGFLESEAYAENGSVAWVTHPELPDHPDHRLRAQLLPHGAGSIISFGIKGGREA
GRRFIEKLALFSHLANVGDAKSLVIHPASTTHAQLDAEALAAAGVGEDMIRLSIGLEDCD
DLIDDLRQALRAATKA