Protein Info for AZOBR_RS11090 in Azospirillum brasilense Sp245

Annotation: alanyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00344: alanine--tRNA ligase
  • amino acids 6 to 866 (861 residues), 974.2 bits, E=3.3e-297
• PF01411: tRNA-synt_2c
  • amino acids 6 to 557 (552 residues), 731.5 bits, E=9.7e-224
• PF07973: tRNA_SAD
  • amino acids 654 to 705 (52 residues), 56.6 bits, 3e-19
• PF02272: DHHA1
  • amino acids 742 to 883 (142 residues), 78.9 bits, E=8.2e-26

Best Hits

Swiss-Prot: 68% identical to SYA_GRABC: Alanine--tRNA ligase (alaS) from Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 91% identity to azl:AZL_a04560)

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• tRNA charging (18/21 steps found)
• enterobactin biosynthesis (6/11 steps found)
• superpathway of chorismate metabolism (40/59 steps found)
• shinorine biosynthesis (3/8 steps found)
• bacillibactin biosynthesis (5/12 steps found)
• ergotamine biosynthesis (4/12 steps found)
• vanchrobactin biosynthesis (1/8 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (4/25 steps found)
• phosphinothricin tripeptide biosynthesis (4/25 steps found)
• corallopyronin A biosynthesis (2/30 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.7

Sequence Analysis Tools

See G8ANE1 at UniProt or InterPro

Protein Sequence (889 amino acids)

>AZOBR_RS11090 alanyl-tRNA synthetase (Azospirillum brasilense Sp245)
MKTANDIRRTFLDFFAKNGHQIVESSPLVPRNDPTLMFTNAGMVQFKNVFTGAEQRPYSR
ATTSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKDDAIAFAWNLVTKEFGL
PADKLLVTVHSSDEDAAGIWRKVAGLSDDRIIRIPTDDNFWRMGDTGPCGPCSEIFFDHG
PHIAGGPPGSPDQDGDRFIEIWNLVFMQYEQRGPDDLIALPKPSIDTGMGLERLAAVLQG
KHDNYDIDLMRALIVASAEATKTAPDGPHAVSHRVIADHLRSCCFLIADGVLPSNEGRGY
VLRRIMRRAMRHAHMIGAREPLMHRLVPALIQQMGDAYPELNRARALIVETLKLEETRFK
QTLERGLRLLEDEVGRLGEGQPLPGDVAFKLYDTYGFPLDLTQDVLRTQGRPVDESGFKA
AMDEQRRKARESWAGSGEATTEKLWYELKDELGATEFFGYDTEVAEGKVTAIVKGDARVE
QATVGDEVLVIVNQTPFYGESGGQVGDAGVIFSAEGTEVAVSDTQKKLGAVWAHVGTVTK
GTLKVGDVVELRVDTERRSAIRANHSATHLLHEALRRRLGEHVTQKGSLVAQERLRFDIS
QPTGLTPADIAAVEDEVNRRILGNSEVVTRLMSPDEARASGAMALFGEKYGDEVRVVSMG
GPHGELDRDYSIELCGGTHVRRTGDIGLFTIVAEGAVAAGVRRIEALTGTGAKAWLSERD
HLLTEAASVLKVRPEDVPSRLAALVDERKKMERELAELRRQVAMGGGAKADGGNEAKDIA
GVKFAARVVEGLPAKDLKPMADELKKQVGSGVVVLIASNEGKASIVVGVTDDLTAKISAV
DLVRVGAEALGGKGGGGRPDMAQAGGPDAALAPAAVEAIEKALAAKAAS