Protein Info for AZOBR_RS10510 in Azospirillum brasilense Sp245

Annotation: amP-dependent synthetase and ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 532 PF00501: AMP-binding" amino acids 18 to 373 (356 residues), 232.6 bits, see alignment E=6.9e-73 PF13193: AMP-binding_C" amino acids 433 to 510 (78 residues), 48.9 bits, see alignment E=1e-16

Best Hits

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AN11 at UniProt or InterPro

Protein Sequence (532 amino acids)

>AZOBR_RS10510 amP-dependent synthetase and ligase (Azospirillum brasilense Sp245)
MYPHLRNEARWVLPEVLAHQAAERGGKTFVTMIAGKENNGDSLTYAEAQEQAGRVAGFFA
GLGVKPGDTVAVMLPNGLDFVRVWLGLGRLGAVMVALNTELKGGFLEHQLENAGAALAVV
DAALADRITDITPRLTALRGLVVPGAAQGSPHTALESWRDAAPYDGPLPRAGDDACIMYT
SGTTGLSKGVLMPHGHGFLFGLGSIDNFALTDEDRFYICLPLFHANGLYMQLYAAMISGG
SAVLRERFSASSWLDDIRRHGCTVTNSLGAVTAFVVAQPPRPDDRDHPLRLVGAAPNPAD
TERVLRERFGVRDVISLYGMTEVNIPLYAEMGKPRPGTCGKVYDRYFDVEIRDPQTDLPV
PRGQVGEVMVRPKAAFGFLSGYHRMPDKTVEAWRNLWFHTGDAAVMDAEGYVTFVDRIKD
CIRRRGENVSSYEVETVLSRLEGVAEVAAYAVPAGIPGAEDEIMVALVAAPGATLTPEAV
AAFAERELPRYAQPRYIDIVDAFPKTPTAKVQKAKLRERGVADTTWDRTKVT