Protein Info for AZOBR_RS10505 in Azospirillum brasilense Sp245

Annotation: ribonuclease H

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 144 PF00075: RNase_H" amino acids 1 to 135 (135 residues), 176.7 bits, see alignment E=1.5e-56

Best Hits

Swiss-Prot: 73% identical to RNH_CHESB: Ribonuclease H (rnhA) from Chelativorans sp. (strain BNC1)

KEGG orthology group: K03469, ribonuclease HI [EC: 3.1.26.4] (inferred from 86% identity to azl:AZL_015380)

MetaCyc: 74% identical to ribonuclease HI (Escherichia coli K-12 substr. MG1655)
Ribonuclease H. [EC: 3.1.26.4]

Predicted SEED Role

"Ribonuclease HI (EC 3.1.26.4)" in subsystem Ribonuclease H (EC 3.1.26.4)

Isozymes

Compare fitness of predicted isozymes for: 3.1.26.4

Use Curated BLAST to search for 3.1.26.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AN10 at UniProt or InterPro

Protein Sequence (144 amino acids)

>AZOBR_RS10505 ribonuclease H (Azospirillum brasilense Sp245)
IFTDGACSGNPGPGGWGAILRYNGVEKELYGGEPATTNNRMELMAAIQALEALKRPMEVR
LYTDSEYVKNGITQWIHGWKARGWKTADKKPVKNEDLWRRLDEAKRQHRIEFHWVRGHAG
HPENERADALARQGVAEVRQTGRG