Protein Info for AZOBR_RS07845 in Azospirillum brasilense Sp245
Annotation: glycerol dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 67% identical to GLDA_THEMA: Glycerol dehydrogenase (gldA) from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
KEGG orthology group: K00005, glycerol dehydrogenase [EC: 1.1.1.6] (inferred from 66% identity to spq:SPAB_04384)MetaCyc: 58% identical to L-1,2-propanediol dehydrogenase / glycerol dehydrogenase (Escherichia coli K-12 substr. MG1655)
(R)-aminopropanol dehydrogenase. [EC: 1.1.1.75]; Glycerol dehydrogenase. [EC: 1.1.1.75, 1.1.1.6]; RXN-8632 [EC: 1.1.1.75, 1.1.1.6]
Predicted SEED Role
"Glycerol dehydrogenase (EC 1.1.1.6)" in subsystem Respiratory dehydrogenases 1 (EC 1.1.1.6)
MetaCyc Pathways
- glycerol degradation II (2/2 steps found)
- methylglyoxal degradation III (2/2 steps found)
- acetone degradation III (to propane-1,2-diol) (1/2 steps found)
- glycerol degradation V (1/2 steps found)
- superpathway of methylglyoxal degradation (5/8 steps found)
- aminopropanol phosphate biosynthesis II (2/4 steps found)
- superpathway of glycerol degradation to 1,3-propanediol (2/4 steps found)
- glycerol degradation to butanol (10/16 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.1.1.6 or 1.1.1.75
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See G8AL93 at UniProt or InterPro
Protein Sequence (364 amino acids)
>AZOBR_RS07845 glycerol dehydrogenase (Azospirillum brasilense Sp245) MITTAIFPARYVQGNGALDSLGEDLARLGKTVLAVTDSFVLKTLKGRLVEAAAGRVEMNI QEFRGECSDEEIERLTGLARAMGADVVAGIGGGKALDTAKAVAHALNARTAIVPTLASTD APCSALSVIYTPEGAFKRYLVLPRNPDLVLVDTGVVASAPVRFLVSGMGDALSTWFEAED CRIKRGGNMTGRVGPMTAFALARLCYDTLLEYGVLAKSACEQGVVTPALERIVEANTLLS GLGFESGGLAAAHAVHNGLTALEETHHCWHGEKVAFGTLTLLILTDRPPAVLDEVYGFCK AVGLPTTLAEIGLAGVSDERLLLAAERACAEGETIHNEPHEITPQRVLAAMKAADAEGRR RKGL