Protein Info for AZOBR_RS07810 in Azospirillum brasilense Sp245
Annotation: poly(3-hydroxyalkanoate) synthetase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 56% identical to PHAC_AZOC5: Poly(3-hydroxyalkanoate) polymerase subunit PhaC (phaC) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)
KEGG orthology group: K03821, polyhydroxyalkanoate synthase [EC: 2.3.1.-] (inferred from 88% identity to azl:AZL_a02630)Predicted SEED Role
"Polyhydroxyalkanoic acid synthase" in subsystem Polyhydroxybutyrate metabolism
KEGG Metabolic Maps
- 1- and 2-Methylnaphthalene degradation
- Alkaloid biosynthesis I
- Alkaloid biosynthesis II
- Anthocyanin biosynthesis
- Benzoate degradation via CoA ligation
- Biosynthesis of terpenoids and steroids
- Biosynthesis of type II polyketide backbone
- Biosynthesis of unsaturated fatty acids
- Butanoate metabolism
- Carotenoid biosynthesis - General
- Diterpenoid biosynthesis
- Ether lipid metabolism
- Ethylbenzene degradation
- Fatty acid biosynthesis
- Glycerophospholipid metabolism
- Glycosphingolipid biosynthesis - ganglio series
- Histidine metabolism
- Limonene and pinene degradation
- Lipopolysaccharide biosynthesis
- Lysine degradation
- Phenylalanine metabolism
- Tyrosine metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.-
Use Curated BLAST to search for 2.3.1.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See G8AL85 at UniProt or InterPro
Protein Sequence (599 amino acids)
>AZOBR_RS07810 poly(3-hydroxyalkanoate) synthetase (Azospirillum brasilense Sp245) MAENQAPDVKLPDPVEMSRAMTRIAEQSQRLVTEFLSRQASDGVGAKNPDPMGVGHAFLE MTTRMMADPAKLMKAQMTLWQDYLTLWQRTTQRFFGQEAQPVIAPAKDDRRFKDSAWDEN TLFDFIKQSYLLSARWMQSTVNEVDGLDDHTAKKVDFYTRQFVDAMAPSNFVMTNPEVLR TTIETGGENLVKGLEHLLKDLERGKGELRISMTDYDAFQVGKNIAVTPGKVVFQTDLMQL IQYTPTTPEVNKRPLMIVPPWINKYYILDLREKNSFIKWAVDQGHSVFVLSWVNPDEKLA QKGFEDYMFEGVLAALDAIEKVTGEKDVNAIGYCLGGTLLASTLSYMAAKKDDRIKSATF FTTMLDFTEAGELSVFIDEEQLTMIESQMAQQGYLDGSKMATTFNMLRANDLIWSFVVNN YLLGKDPFPFDLLYWNSDSTRMPAAMHSFYLRNMYQKNLLAKPGAVSLGGVPIDLRNVKT PSFFLSAREDHIAPWKSTYMGAHLFSGPVKFVLAASGHIAGVVNPPAAGKYCYWTNAKLP KASDDWLASSEQTPGSWWPEWNSWVSTFSEGKVPARNPEKGGLPVLEDAPGSYAKVRIV